Role proteinů FcRL1 a FcRL3 během vazby a fúze membrán gamet.

Název projektu anglicky
The role of FcRL1 and FcRL3 proteins during gamete membrane binding and fusion.
Anotace anglicky
Prior research has identified molecular changes that gametes undergo during fertilization. So far, three main proteins (IZUMO1, CD9 and JUNO) have been known as essential for gamete fusion, whereas the sperm fusion receptor on the oocyte has not been identified and may be species specific. We aim to investigate the role of FcRL1 and FcRL3, localized on the oolemma, as novel candidates for fusion proteins involved in human fertilization. We intend to carry out a comprehensive study deciphering FcRL1 and FcRL3 cis-interactions with oocyte JUNO and tetraspanins CD9/CD81, and trans-interactions with sperm IZUMO1. We will use transfected cell lines and generate mice with conditional expression of the human hFcrl3/hFcrl1 genes upon CRE-mediated recombination for tissue specific overexpression. We will compare the species specificity of FcRL receptors using a newly generated orthologue genes double knock-out mouse model to study the phenotype in vivo. The project will bring highly innovative results that will broaden our knowledge concerning gamete membrane binding and fusion.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10604 - Reproductive biology (medical aspects to be 3)
OECD FORD - vedlejší obor
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OECD FORD - další vedlejší obor
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CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
EB - Genetika a molekulární biologie

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP25-GA0-GA-R
Datum dodání záznamu
12. 3. 2025

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