Interakce nanočástic s biomolekulami - vznik proteinové korony, její vliv na vstup nanočástic do buňky a na sekundární toxicitu nanočástic.

Název projektu anglicky
Protein corona formation on nanopaticles and its influence on entry of nanoparticles into cell and nanoparticles secondary toxicity.
Anotace anglicky
When nanoparticles enter a cell or a living organism, biomolecules adhere to their surface and form a so-called protein corona. This affects not only the entry of nanoparticles into the cell but also the overall toxicity of nanoparticles and their fate in the cell. The secondary toxicity of nanoparticles caused by the interaction of molecules on their surface greatly limits their use in medicine and pharmacy. This issue has so far been studied by outdated indirect ensemble methods. Our approach examines individual nanoparticles and is focused on the rate of protein corona formation and its stability by studying individual anchored nanoparticles under liquid using modified atomic force microscopy and cryo-TEM technique. The conformational change of the proteins will be further monitored by vibrational spectroscopy. We will also investigate the entry of nanoparticles with/ without protein corona into cells using these techniques and their influence on the mechano-chemical properties of the cell membrane. Au and Si-nanoparticles with sizes of 1-10 nm and 100 nm will serve as main models

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10610 - Biophysics
OECD FORD - vedlejší obor
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OECD FORD - další vedlejší obor
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CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
BO - Biofyzika

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP24-GA0-GA-R
Datum dodání záznamu
27. 2. 2024

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