Bridging Structural and Cellular Biology: Protein in-cell NMR Spectroscopy in 3D Stem Cell-Derived Human Organ Models

Název projektu anglicky
Bridging Structural and Cellular Biology: Protein in-cell NMR Spectroscopy in 3D Stem Cell-Derived Human Organ Models
Anotace anglicky
In-cell NMR spectroscopy is the only analytical method that enables the structural characterization of proteins and their interactions in living cells at atomic resolution. It has recently emerged as a unique tool for addressing various research questions related to human physiology and drug development. Its applications include target structure validation, drug-target engagement assessment, and evaluation of drug-target affinity under native-like conditions. However, in-cell NMR spectroscopy in human cells has been limited to asynchronous single-cell(-type) cultures. Unfortunately, these cultures do not reliably recapitulate the key functional properties that cells exhibit when part of a tissue. Additionally, many cell types cannot be maintained in traditional culture conditions but can only be propagated when differentiated in situ from progenitor (pluripotent stem) cells as part of a human tissue-like environment. Reliance on single-cell(-type) cultures represents a major limitation of current in-cell NMR applications in drug development and basic research.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10406 - Analytical chemistry
OECD FORD - vedlejší obor
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OECD FORD - další vedlejší obor
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CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
CB - Analytická chemie, separace

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP26-GA0-GA-R
Datum dodání záznamu
29. 4. 2026

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