Distal and proximal controllers of gene activity in barley

Název projektu anglicky
Distal and proximal controllers of gene activity in barley
Anotace anglicky
Gene transcription is controlled by an interplay of transcription factors and cis-regulatory elements (CREs) – promoters, enhancers and silencers. A growing number of studies point to an association between the CREs and advantageous traits in crops, reinforcing interest in these elements. Previously, we intersected data from epigenome and interactome profiling in barley embryo and predicted over 2000 putative distal CREs and their target genes. Here, we plan to assess possible impact of these CREs on the transcription of their targets by comparing data from barley pan-genome and pan-transcriptome. Promising CREs will be validated by a luciferase reporter assay. Moreover, CREs of two developmentally regulated genes affecting agronomically important traits – GTL1 and SWEET11b - will be validated by CRISPR-Cas editing. Finally, to assess a predicted upstream regulator of SWEET11b, we will remove its binding site from the SWEET11b promoter. Results of this project will increase the knowledge of cis-regulation in plants and provide possible targets for bioengineering in cereal crops.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10603 - Genetics and heredity (medical genetics to be 3)
OECD FORD - vedlejší obor
10605 - Developmental biology
OECD FORD - další vedlejší obor
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CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
EB - Genetika a molekulární biologie

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP26-GA0-GA-R
Datum dodání záznamu
30. 4. 2026

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