Výzkum mechanismů signalizace G proteinu β2-adrenergním receptorem

Název projektu anglicky
Insights into mechanisms of G protein signaling by the β2-adrenergic receptor
Anotace anglicky
The beta2-adrenergic receptor (b2AR) is the archetypal G protein coupled receptor. Signaling through the b2AR is crucial to the function of many organs, and has been linked to such diseases as heart failure, asthma and glaucoma. However, despite a number of studies, many mechanistic aspects of b2AR signaling remain unclear. Of high scientific and practical interest is b2AR’s ability to couple both to stimulatory Gs and inhibitory Gi proteins, with opposite effects on cell homeostasis. We propose to utilize the unique capabilities of two-photon polarization microscopy (2PPM), a technique recently developed in our lab, to address several important questions on b2AR signaling through Gs and Gi. Namely, we propose to determine whether non-stimulated b2AR forms stable complexes with either Gs or Gi, and ascertain whether the heterotrimers of these G proteins dissociate into free Ga and Gbg subunits or rearrange upon activation by the b2AR. These findings will shed light on the mechanism of dual coupling of b2AR and the interplay of the Gs and Gi proteins in cellular signal transduction.

Kategorie VaV
ZV - Základní výzkum
CEP - hlavní obor
ED - Fyziologie
CEP - vedlejší obor
FR - Farmakologie a lékárnická chemie
CEP - další vedlejší obor
CE - Biochemie
OECD FORD - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
10608 - Biochemistry and molecular biology<br>10609 - Biochemical research methods<br>30104 - Pharmacology and pharmacy<br>30105 - Physiology (including cytology)

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP21-GA0-GJ-R/02:1
Datum dodání záznamu
22. 2. 2021

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