Efektivní detekce hostitelů a kultivace sladkovodních CPR bakteríí (‘Ca. Patescibacteria’)

Název projektu anglicky
High-throughput host detection and cultivation of freshwater CPRs (‘Ca. Patescibacteria’)
Anotace anglicky
One of the most important discoveries in microbiology in the last decade was Candidate Phyla Radiation (CPR) or ‘Ca. Patescibacteria’, a recently described phylum of mostly episymbionts that include up to 26% of bacterial diversity. Although >2400 representative genomes are available, less than a dozen members were cultivated to date, belonging to just two out of 24 CPR classes. Because to their prevalence in the environment, cultivating ‘Ca. Patescibacteria’ is important, but notoriously difficult due to their complex nutrition needs and reliance on other microorganisms, and new strategies and techniques are needed to understand their ecophysiology. In our project, we propose to target both partners with metatranscriptomics and EpicPCR to assess the functional potential and host range of CPRs. Further, we aim to obtain episymbionts - hosts co-cultures by combining reverse metagenomics and high-throughput cultivation. These approaches represent the necessary steps that will advance the understanding of CPRs biology.

Kategorie VaV
ZV - Základní výzkum
OECD FORD - hlavní obor
10617 - Marine biology, freshwater biology, limnology
OECD FORD - vedlejší obor
10618 - Ecology
OECD FORD - další vedlejší obor
10606 - Microbiology
CEP - odpovídající obory <br>(dle <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">převodníku</a>)
DA - Hydrologie a limnologie<br>EE - Mikrobiologie, virologie<br>EH - Ekologie – společenstva

Důvěrnost údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Systémové označení dodávky dat
CEP25-GA0-GM-R
Datum dodání záznamu
21. 2. 2025

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