Filtry
Cytoplasmic helicase encoded by pGKL plasmids
For production of putative helicase we used T7-based system and despite of difficulty with production of AT rich sequences we successfully expressed ORF4p helicase in E. coli. Further, we partly purified ORF4p helicase by a...
EB - Genetika a molekulární biologie
- 2003 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Vector encoding gene for expression of RNA helicase in E. coli, originating from linear plasmids of the yeast Kluyveromyces lactis
An expression vector carrying gene for RNA helicase encoded by linear plasmids of Kluyveromyces lactis (ORF4-pGKL2) was prepared. The sequence encoding RNA helicase was optimized for heterologous expression in E.coli. RNA helica...
Biological sciences
- 2024 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
The Localization of Nuclear DNA Helicase II in Different Nuclear Compartments Is Linked to Transcription.
Nuclear DNA helicase II (DNH II) is a member of the DEAH superfamily of helicases and functions as a premRNA- and mRNA-binding protein in human cells. Here we report for the first time that human NDH II is associated with the nucleo...
EB - Genetika a molekulární biologie
- 2002 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Nuclear DNA helicase II is recruited to IFN-alpha-activated transcription sites at PML nuclear bodies
It is known that nuclear helicase II links CREB-binding protein directly to RNA polymerase II holoenzyme.
CE - Biochemie
- 2002 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Nuclear DNA helicase II is recruited to IFN-α-activated transcription sites at PML nuclear bodies.
We report for the first time that some NDH II/RNA helicase A is a component of promyelocytic leukemia nuclear bodies (PML NBs). We suggest that DNH II recruitment to PML NBs is connected with transcriptional regulation of interferon-α-i...
EB - Genetika a molekulární biologie
- 2002 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
HEK-293 cell line expressing DHX15 RNA helicase tagged with mScarlet fluorescent protein
Using CRISPR-Cas9 technology, a cell line expressing the RNA helicase DHX15 tagged with the fluorescent protein mScarlet was created. The RNA helicase DHX15 is crucial for the replication of certain retroviruses, playing a role in t...
Microbiology
- 2024 •
- Gfunk
Rok uplatnění
Gfunk - Funkční vzorek
Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.
yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also and is particularlyadept at unwinding these structures. ...
EB - Genetika a molekulární biologie
- 2009 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Distinct functions of human RecQ helicases during DNA replication
that maintain genomic stability. Human RecQ helicases are among the most important factors of replication stress. RecQ helicases promote recovery of replication forks being stalled due, and are involved in proper checkpoint signali...
Biochemistry and molecular biology
- 2017 •
- Jimp •
- Odkaz
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities
helicases capable of preventing excessive recombination. In this study, we show that the human Fbh1 (hFbh1) helicase accumulates at sites of DNA damage or replication stress in a manner dependent fully on its helicase acti...
EB - Genetika a molekulární biologie
- 2009 •
- Jx
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
The human RecQ helicases BLM and RECQL4 cooperate to preserve genome stability
Bacteria and yeast possess one RecQ helicase homolog whereas humans contain five RecQ helicases, all of which are important in preserving genome stability helicases function cooperatively. Here, we report a novel physical a...
EB - Genetika a molekulární biologie
- 2012 •
- Jx •
- Odkaz
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
- 1 - 10 z 166