Protocol for efficient micropropagation of spring gentian and sand jurinea
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F14%3A%230000817" target="_blank" >RIV/00020702:_____/14:#0000817 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.agriculturejournals.cz/publicFiles/112132.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/112132.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Protocol for efficient micropropagation of spring gentian and sand jurinea
Popis výsledku v původním jazyce
Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1 of glutamine, 200 mg.l-1 of casein hydrolysate, 30 g.l-1 of sucrose, 0.2 mg.l-1 of BAP, and o.1 mg.l-1 of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg.l-1 of IBA 70-75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J.
Název v anglickém jazyce
Protocol for efficient micropropagation of spring gentian and sand jurinea
Popis výsledku anglicky
Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1 of glutamine, 200 mg.l-1 of casein hydrolysate, 30 g.l-1 of sucrose, 0.2 mg.l-1 of BAP, and o.1 mg.l-1 of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg.l-1 of IBA 70-75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EF - Botanika
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Forest Science
ISSN
1212-4834
e-ISSN
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Svazek periodika
60
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
5
Strana od-do
1-5
Kód UT WoS článku
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EID výsledku v databázi Scopus
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