Use of nuclear microsatellite loci for evaluating genetic diversity among selected populations of Abies alba Mill. in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F15%3A%230000911" target="_blank" >RIV/00020702:_____/15:#0000911 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.agriculturejournals.cz/publicFiles/160212.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/160212.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/25/2015-JFS" target="_blank" >10.17221/25/2015-JFS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Use of nuclear microsatellite loci for evaluating genetic diversity among selected populations of Abies alba Mill. in the Czech Republic

Popis výsledku v původním jazyce

The simple sequence repeats (SSR) method of DNA analysis was used to determine genetic diversity within and among silver fir (Abies alba Mill.) populations. Total genomic DNA was extracted from 250 Abies alba adult individuals collected from 8 important populations growing in different parts of the Czech Republic. Samples were screened using eight selected polymorphic nuclear microsatellite markers. PCR products were separated by capillary electrophoresis. Identified genetic loci were verified as reliable and polymorphic and were used for comparative genetic analyses of silver fir populations. The genotypic data from the adult trees implies genetic differences among the studied populations. The populations’ genetic diversity expressed as Shannon’s information index values was highest in the JD06 population from Hochwald. The lowest genetic diversity was in the JD02 population from Velké Polčané, Morávka. The longest genetic distance appeared between the JD02 (Velké Polčané, Morávka) and JD07 (Hojsova Stráž) populations. The closest genetic distance was between the JD01 (Babín) and JD03 (Vodslivy) populations.

Název v anglickém jazyce

Use of nuclear microsatellite loci for evaluating genetic diversity among selected populations of Abies alba Mill. in the Czech Republic

Popis výsledku anglicky

The simple sequence repeats (SSR) method of DNA analysis was used to determine genetic diversity within and among silver fir (Abies alba Mill.) populations. Total genomic DNA was extracted from 250 Abies alba adult individuals collected from 8 important populations growing in different parts of the Czech Republic. Samples were screened using eight selected polymorphic nuclear microsatellite markers. PCR products were separated by capillary electrophoresis. Identified genetic loci were verified as reliable and polymorphic and were used for comparative genetic analyses of silver fir populations. The genotypic data from the adult trees implies genetic differences among the studied populations. The populations’ genetic diversity expressed as Shannon’s information index values was highest in the JD06 population from Hochwald. The lowest genetic diversity was in the JD02 population from Velké Polčané, Morávka. The longest genetic distance appeared between the JD02 (Velké Polčané, Morávka) and JD07 (Hojsova Stráž) populations. The closest genetic distance was between the JD01 (Babín) and JD03 (Vodslivy) populations.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1230334" target="_blank" >QJ1230334: Ověření geneticky podmíněné proměnlivosti významných populací lesních dřevin, včetně genetické inventarizace vybraných ekotypů, jako podklad pro aktualizaci souvisejících legislativních předpisů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Forest Science

ISSN

1212-4834

e-ISSN

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Svazek periodika

61

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

345-351

Kód UT WoS článku

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EID výsledku v databázi Scopus

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