Use of nuclear microsatellite loci for evaluating genetic diversity of selected populations of Picea abies (L.) Karsten in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F18%3AN0000076" target="_blank" >RIV/00020702:_____/18:N0000076 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/1999-4907/9/2/92" target="_blank" >https://www.mdpi.com/1999-4907/9/2/92</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/f9020092" target="_blank" >10.3390/f9020092</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Use of nuclear microsatellite loci for evaluating genetic diversity of selected populations of Picea abies (L.) Karsten in the Czech Republic

Popis výsledku v původním jazyce

DNA polymorphism at nine nuclear microsatellites of nine selected naturally-regenerated Norway spruce populations growing mainly within gene conservation units in different parts of the Czech Republic was studied. To verify the genetic quality of the selected gene conservation unit, we analyzed nine Norway spruce subpopulations from gene conservation unit GZ 102-Orlicke hory. Genetic parameters can be used in state administrative decision making on including stands into gene conservation units. The level of genetic diversity within 17 investigated Czech Norway spruce units was relatively high. Mean values for the number of different alleles ranged from 12.2 (population SM 08) to 16.2 (subpopulation SM T4). The values of observed heterozygosity (H-o) ranged from 0.65 to 0.80 and expected heterozygosity (H-e) from 0.74 to 0.81. Pairwise population F-ST values ranging from 0.006 to 0.027 indicated low genetic differentiation between units, and values of Nei's genetic distance among Norway spruce units ranged from 0.046 to 0.168, thus structuring of the investigated Norway spruce units was confirmed. Closer genetic similarity was seen in subpopulations from the gene conservation unit in Orlicke hory than in the studied populations from other genetic conservation units. Additionally, the populations SM 01 and SM 05, both of Hurst ecotypes, were the closest to one another and the populations of mountain and alpine ecotypes were assembled into another group.

Název v anglickém jazyce

Use of nuclear microsatellite loci for evaluating genetic diversity of selected populations of Picea abies (L.) Karsten in the Czech Republic

Popis výsledku anglicky

DNA polymorphism at nine nuclear microsatellites of nine selected naturally-regenerated Norway spruce populations growing mainly within gene conservation units in different parts of the Czech Republic was studied. To verify the genetic quality of the selected gene conservation unit, we analyzed nine Norway spruce subpopulations from gene conservation unit GZ 102-Orlicke hory. Genetic parameters can be used in state administrative decision making on including stands into gene conservation units. The level of genetic diversity within 17 investigated Czech Norway spruce units was relatively high. Mean values for the number of different alleles ranged from 12.2 (population SM 08) to 16.2 (subpopulation SM T4). The values of observed heterozygosity (H-o) ranged from 0.65 to 0.80 and expected heterozygosity (H-e) from 0.74 to 0.81. Pairwise population F-ST values ranging from 0.006 to 0.027 indicated low genetic differentiation between units, and values of Nei's genetic distance among Norway spruce units ranged from 0.046 to 0.168, thus structuring of the investigated Norway spruce units was confirmed. Closer genetic similarity was seen in subpopulations from the gene conservation unit in Orlicke hory than in the studied populations from other genetic conservation units. Additionally, the populations SM 01 and SM 05, both of Hurst ecotypes, were the closest to one another and the populations of mountain and alpine ecotypes were assembled into another group.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

<a href="/cs/project/QJ1530294" target="_blank" >QJ1530294: Modelový postup molekulárně genetické charakterizace genové základny jako podklad pro účely rozhodování státní správy v oblasti zachování a reprodukce genetických zdrojů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Forests

ISSN

1999-4907

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

Article Number 92

Kód UT WoS článku

000427520600045

EID výsledku v databázi Scopus

2-s2.0-85042182656