Long-term observation of in vitro-derived Malus sylvestris (L.) Mill., the path from the bud to the tree

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F23%3AN0000093" target="_blank" >RIV/00020702:_____/23:N0000093 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.intechopen.com/chapters/84344" target="_blank" >https://www.intechopen.com/chapters/84344</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5772/intechopen.108359" target="_blank" >10.5772/intechopen.108359</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Long-term observation of in vitro-derived Malus sylvestris (L.) Mill., the path from the bud to the tree

Popis výsledku v původním jazyce

The European wild apple (Malus sylvestris L.), a wild contributor to the domesticated apple, belongs to the endangered species in the Czech Republic. Thus, an efficient protocol was developed for in vitro plantlet regeneration using the post-dormant buds. The highest shoot induction was obtained on MS medium supplemented with 0.5 mg.l−1 BAP, 5 mg.l−1 GA and 0.1 mg.l−1 IBA. Shoot multiplication and elongation took place on the same medium with 0.2 mg.l−1 BAP and 0.1 mg.l−1 IBA. Indole-3-butyric acid at 0.5 mg.l−1 was most effective for rooting. The micropropagated plantlets were successfully acclimatized in greenhouse conditions and were transplanted into soil in forest. Finally, qualitative and quantitative parameters of tissue culture-derived plants were evaluated. Monitoring of in vitro plantings on experimental trials suggests that micropropagated wild apple trees retain the growth characteristics of generative individuals.

Název v anglickém jazyce

Long-term observation of in vitro-derived Malus sylvestris (L.) Mill., the path from the bud to the tree

Popis výsledku anglicky

The European wild apple (Malus sylvestris L.), a wild contributor to the domesticated apple, belongs to the endangered species in the Czech Republic. Thus, an efficient protocol was developed for in vitro plantlet regeneration using the post-dormant buds. The highest shoot induction was obtained on MS medium supplemented with 0.5 mg.l−1 BAP, 5 mg.l−1 GA and 0.1 mg.l−1 IBA. Shoot multiplication and elongation took place on the same medium with 0.2 mg.l−1 BAP and 0.1 mg.l−1 IBA. Indole-3-butyric acid at 0.5 mg.l−1 was most effective for rooting. The micropropagated plantlets were successfully acclimatized in greenhouse conditions and were transplanted into soil in forest. Finally, qualitative and quantitative parameters of tissue culture-derived plants were evaluated. Monitoring of in vitro plantings on experimental trials suggests that micropropagated wild apple trees retain the growth characteristics of generative individuals.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Apple cultivation – recent advances

ISBN

978-1-83969-800-2

Počet stran výsledku

14

Strana od-do

25-38

Počet stran knihy

130

Název nakladatele

Intech

Místo vydání

London

Kód UT WoS kapitoly

—