Possibilities for reproduction and archiving resulting in a clone collection of a unique grey poplar (Populus × canescens Aiton Sm.) population in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F24%3AN0000044" target="_blank" >RIV/00020702:_____/24:N0000044 - isvavai.cz</a>

Výsledek na webu

<a href="https://sciendo.com/article/10.2478/forj-2024-0005" target="_blank" >https://sciendo.com/article/10.2478/forj-2024-0005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/forj-2024-0005" target="_blank" >10.2478/forj-2024-0005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Possibilities for reproduction and archiving resulting in a clone collection of a unique grey poplar (Populus × canescens Aiton Sm.) population in the Czech Republic

Popis výsledku v původním jazyce

Floodplain forests as one of the most endangered ecosystems in Europe have recently been impacted by fungal pathogens Phytophtora sp. and Hymenoscyphus fraxineus (chalara). Grey poplar (Populus x canescens Aiton Sm.) can be used as one of well adapted tree species for the reforestation of withering stands of ash (Fraxinus sp.). A unique population of grey poplar (Populus x canescens Aiton Sm.) characterized by desirable phenotypic traits was used for this purpose. The gender distribution was asymmetric; out of 155 individuals, 113 were female. Out of 33 different genotypes determined in the population, 15 were used as a source of approved forest reproductive material. Vegetative reproduction methods (ex situ clonal reproduction by micropropagation and cuttings) were developed and used, to rapidly initiate the recovery of forest stands of grey poplar. In total, 940 explants were successfully micropropagated and adapted to natural conditions, to ensure a genetically diverse source of viable plants used for reforestation. Moreover, we used methodological procedures of micropropagation for setting up cryopreservation technique. With respect to long-term storage of valuable grey poplar genotypes, modulation of gene expression by cold hardening during cryopreservation revealed significant changes in a few candidate genes involved in plant cellular processes.

Název v anglickém jazyce

Possibilities for reproduction and archiving resulting in a clone collection of a unique grey poplar (Populus × canescens Aiton Sm.) population in the Czech Republic

Popis výsledku anglicky

Floodplain forests as one of the most endangered ecosystems in Europe have recently been impacted by fungal pathogens Phytophtora sp. and Hymenoscyphus fraxineus (chalara). Grey poplar (Populus x canescens Aiton Sm.) can be used as one of well adapted tree species for the reforestation of withering stands of ash (Fraxinus sp.). A unique population of grey poplar (Populus x canescens Aiton Sm.) characterized by desirable phenotypic traits was used for this purpose. The gender distribution was asymmetric; out of 155 individuals, 113 were female. Out of 33 different genotypes determined in the population, 15 were used as a source of approved forest reproductive material. Vegetative reproduction methods (ex situ clonal reproduction by micropropagation and cuttings) were developed and used, to rapidly initiate the recovery of forest stands of grey poplar. In total, 940 explants were successfully micropropagated and adapted to natural conditions, to ensure a genetically diverse source of viable plants used for reforestation. Moreover, we used methodological procedures of micropropagation for setting up cryopreservation technique. With respect to long-term storage of valuable grey poplar genotypes, modulation of gene expression by cold hardening during cryopreservation revealed significant changes in a few candidate genes involved in plant cellular processes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

<a href="/cs/project/QK1910277" target="_blank" >QK1910277: Využití metody kryoprezervace pro zefektivnění šlechtitelského procesu hospodářsky významných zemědělských plodin a uchování lesních dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Central European Forestry Journal

ISSN

2454-034X

e-ISSN

2454-0358

Svazek periodika

70

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

13

Strana od-do

144-155

Kód UT WoS článku

001316643300005

EID výsledku v databázi Scopus

2-s2.0-85204994083