The effect of epigenetic factors on differentiations of pancreatic progenitor cells into insulin-producings cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F11%3A00002530" target="_blank" >RIV/00023001:_____/11:00002530 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0041134511014084" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0041134511014084</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.transproceed.2011.10.025" target="_blank" >10.1016/j.transproceed.2011.10.025</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The effect of epigenetic factors on differentiations of pancreatic progenitor cells into insulin-producings cells
Popis výsledku v původním jazyce
Differentiation of pancreatic progenitors into insulin-producing cells is regulated by various transcription factors. To be expressed the genes coding these transcription factors need to be in accessible DNA. Whether a particular gene is present in a form of active euchromatin structure with accessible DNA or in an inactive heterochromatin structure with inaccessible DNA is determined by various epigenetic modifications. We studied the effect of epigenetic modifiers on differentiation of human nonendocrine cells into insulin-producing cells with the aim to evaluate the effect of epigenetic modifications in that process. Within 3 days of cultivation nonendocrine cells form isletlike cell clusters (ILCCs) containing mainly cytokeratin-19-positive cells.After cultivation with epigenetic modifiers and further differentiation, the highest number of C-peptide-positive cells (10.3% 2.9%) as well as glucagon-positive cells (7.2% 2.8%) was observed in a samplesupplemented with a combination of
Název v anglickém jazyce
The effect of epigenetic factors on differentiations of pancreatic progenitor cells into insulin-producings cells
Popis výsledku anglicky
Differentiation of pancreatic progenitors into insulin-producing cells is regulated by various transcription factors. To be expressed the genes coding these transcription factors need to be in accessible DNA. Whether a particular gene is present in a form of active euchromatin structure with accessible DNA or in an inactive heterochromatin structure with inaccessible DNA is determined by various epigenetic modifications. We studied the effect of epigenetic modifiers on differentiation of human nonendocrine cells into insulin-producing cells with the aim to evaluate the effect of epigenetic modifications in that process. Within 3 days of cultivation nonendocrine cells form isletlike cell clusters (ILCCs) containing mainly cytokeratin-19-positive cells.After cultivation with epigenetic modifiers and further differentiation, the highest number of C-peptide-positive cells (10.3% 2.9%) as well as glucagon-positive cells (7.2% 2.8%) was observed in a samplesupplemented with a combination of
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FB - Endokrinologie, diabetologie, metabolismus, výživa
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Transplantation Proceedings
ISSN
0041-1345
e-ISSN
—
Svazek periodika
43
Číslo periodika v rámci svazku
9
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
5
Strana od-do
3212-3216
Kód UT WoS článku
000297485500017
EID výsledku v databázi Scopus
—