Conditional knockout of integrin alpha 2 beta 1 in murine megakaryocytes leads to reduced mean platelet volume

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F13%3A00057955" target="_blank" >RIV/00023001:_____/13:00057955 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0055094" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0055094</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0055094" target="_blank" >10.1371/journal.pone.0055094</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Conditional knockout of integrin alpha 2 beta 1 in murine megakaryocytes leads to reduced mean platelet volume

Popis výsledku v původním jazyce

We have engineered a transgenic mouse on a C57BL/6 background that bears a floxed Itga2 gene. The crossing of this mouse strain to transgenic mice expressing Cre recombinase driven by the megakaryocyte (MK)-specific Pf4 promoter permits the conditional knockout of Itga2 in the MK/platelet lineage. Mice lacking MK alpha 2 beta 1 develop normally, are fertile, and like their systemic alpha 2 beta 1 knockout counterparts, exhibit defective adhesion to and aggregation induced by soluble type I collagen anda delayed onset to low dose fibrillar collagen-induced aggregation, results consistent with blockade or loss of platelet alpha 2 beta 1. At the same time, we observed a significant reduction in mean platelet volume, which is consistent with the reportedrole of alpha 2 beta 1 in MK maturation and proplatelet formation in vivo. This transgenic mouse strain bearing a floxed Itga2 gene will prove valuable to distinguish in vivo the temporal and spatial contributions of alpha 2 integrin in s

Název v anglickém jazyce

Conditional knockout of integrin alpha 2 beta 1 in murine megakaryocytes leads to reduced mean platelet volume

Popis výsledku anglicky

We have engineered a transgenic mouse on a C57BL/6 background that bears a floxed Itga2 gene. The crossing of this mouse strain to transgenic mice expressing Cre recombinase driven by the megakaryocyte (MK)-specific Pf4 promoter permits the conditional knockout of Itga2 in the MK/platelet lineage. Mice lacking MK alpha 2 beta 1 develop normally, are fertile, and like their systemic alpha 2 beta 1 knockout counterparts, exhibit defective adhesion to and aggregation induced by soluble type I collagen anda delayed onset to low dose fibrillar collagen-induced aggregation, results consistent with blockade or loss of platelet alpha 2 beta 1. At the same time, we observed a significant reduction in mean platelet volume, which is consistent with the reportedrole of alpha 2 beta 1 in MK maturation and proplatelet formation in vivo. This transgenic mouse strain bearing a floxed Itga2 gene will prove valuable to distinguish in vivo the temporal and spatial contributions of alpha 2 integrin in s

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

"e55094"

Kód UT WoS článku

000314023600151

EID výsledku v databázi Scopus

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