Silybin affects the liver microsomal CYP2C6 in HHTg rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F13%3A00058739" target="_blank" >RIV/00023001:_____/13:00058739 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00098892:_____/13:#0000416

Výsledek na webu

<a href="http://biomed.papers.upol.cz/incpdfs/pri-990000-0500_10_031.pdf" target="_blank" >http://biomed.papers.upol.cz/incpdfs/pri-990000-0500_10_031.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Silybin affects the liver microsomal CYP2C6 in HHTg rats

Popis výsledku v původním jazyce

Aim. This study aims to investigate the effect of silybin on liver expression and activity of rat CYP2C6 under mild liver steatosis. This cytochrome P450 enzyme is considered to be a counterpart of human CYP2C9, which metabolizes commonly prescribed drugs, such as ibuprofen, diclofenac, or warfarin. Methods. Male hereditary hypertriglyceridemic rats (accepted model of metabolic syndrome) were fed: 1) standard laboratory diet (STD), 2) high cholesterol diet (HCD = STD + 1% of cholesterol w/w + 10% of lard fat w/w), 3) high cholesterol diet with silybin (0.5% w/w) for 21 days. Expression of cytochrome P450 2C6 was measured in liver using real-time PCR (at mRNA level) and Western blotting (at protein level). Formation of diclofenac metabolite (typical marker substrate of CYP2C6 enzyme activity) was analyzed using HPLC with UV detection. Results. Silybin in hereditary hypertriglyceridemic rats on HCD diet significantly increased activity of CYP2C6 and its expression on mRNA level. Expressi

Název v anglickém jazyce

Silybin affects the liver microsomal CYP2C6 in HHTg rats

Popis výsledku anglicky

Aim. This study aims to investigate the effect of silybin on liver expression and activity of rat CYP2C6 under mild liver steatosis. This cytochrome P450 enzyme is considered to be a counterpart of human CYP2C9, which metabolizes commonly prescribed drugs, such as ibuprofen, diclofenac, or warfarin. Methods. Male hereditary hypertriglyceridemic rats (accepted model of metabolic syndrome) were fed: 1) standard laboratory diet (STD), 2) high cholesterol diet (HCD = STD + 1% of cholesterol w/w + 10% of lard fat w/w), 3) high cholesterol diet with silybin (0.5% w/w) for 21 days. Expression of cytochrome P450 2C6 was measured in liver using real-time PCR (at mRNA level) and Western blotting (at protein level). Formation of diclofenac metabolite (typical marker substrate of CYP2C6 enzyme activity) was analyzed using HPLC with UV detection. Results. Silybin in hereditary hypertriglyceridemic rats on HCD diet significantly increased activity of CYP2C6 and its expression on mRNA level. Expressi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical papers

ISSN

1213-8118

e-ISSN

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Svazek periodika

157

Číslo periodika v rámci svazku

suppl. 1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

"S60"-"S64"

Kód UT WoS článku

000324728600004

EID výsledku v databázi Scopus

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