Reverse transport of cholesterol is the reason for resistance to development of atherosclerosis in Prague Hereditary Hypercholesterolemic (PHHC) Ra

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F14%3A00059074" target="_blank" >RIV/00023001:_____/14:00059074 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.biomed.cas.cz/physiolres/pdf/63/63_591.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/63/63_591.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Reverse transport of cholesterol is the reason for resistance to development of atherosclerosis in Prague Hereditary Hypercholesterolemic (PHHC) Ra

Popis výsledku v původním jazyce

The Prague Hereditary Hypercholesterolemic (PHHC) rat is a model of hypercholesterolemia. In previous experiments, it was found to be completely resistant to the development of atherosclerosis. It was assumed that the reverse transport of cholesterol (RCT) might be the reason for this resistance. In this study, RCT was measured in vivo by cholesterol efflux from macrophages to plasma, using previously established methods for RCT in mice (Rader 2003), optimized for measurements in rats. Primary cell culture of macrophages was labeled with (14)C-cholesterol and then injected intraperitoneally into rats. Plasma and feces were collected at 24 and 48 h. The plasma (14)C-cholesterol levels at both 24 and 48 h were significantly higher in male PHHC rats compared to control Wistar rats. The PHHC rats excreted less (14)C-cholesterol in feces in 24 and 48 h compared to Wistar rats. The largest pool of (14)C-cholesterol was found in the adipose tissue of PHHC rats and in contrast lower levels of

Název v anglickém jazyce

Reverse transport of cholesterol is the reason for resistance to development of atherosclerosis in Prague Hereditary Hypercholesterolemic (PHHC) Ra

Popis výsledku anglicky

The Prague Hereditary Hypercholesterolemic (PHHC) rat is a model of hypercholesterolemia. In previous experiments, it was found to be completely resistant to the development of atherosclerosis. It was assumed that the reverse transport of cholesterol (RCT) might be the reason for this resistance. In this study, RCT was measured in vivo by cholesterol efflux from macrophages to plasma, using previously established methods for RCT in mice (Rader 2003), optimized for measurements in rats. Primary cell culture of macrophages was labeled with (14)C-cholesterol and then injected intraperitoneally into rats. Plasma and feces were collected at 24 and 48 h. The plasma (14)C-cholesterol levels at both 24 and 48 h were significantly higher in male PHHC rats compared to control Wistar rats. The PHHC rats excreted less (14)C-cholesterol in feces in 24 and 48 h compared to Wistar rats. The largest pool of (14)C-cholesterol was found in the adipose tissue of PHHC rats and in contrast lower levels of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FA - Kardiovaskulární nemoci včetně kardiochirurgie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological research

ISSN

0862-8408

e-ISSN

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Svazek periodika

63

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

591-596

Kód UT WoS článku

000345590100009

EID výsledku v databázi Scopus

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