Pre-transplant donor-specific Interferon-gamma-producing cells and acute rejection of the kidney allograft

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F15%3A00059552" target="_blank" >RIV/00023001:_____/15:00059552 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0966327415300125" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0966327415300125</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.trim.2015.07.007" target="_blank" >10.1016/j.trim.2015.07.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Pre-transplant donor-specific Interferon-gamma-producing cells and acute rejection of the kidney allograft

Popis výsledku v původním jazyce

Background: Our retrospective study included a cohort of 47 patients who underwent living donor kidney transplantation. The pre-transplant frequencies of donor-specific Interferon-gamma (IFN-gamma) producing cells were defined using enzyme-linked immunosorbent spot (ELISpot) assay and correlated with incidence of acute cellular (ACR), antibody-mediated rejection (AMR) and kidney graft survival up to one year after transplantation. Results: We found a statistically significant correlation between the frequencies of IFN-gamma-producing cells and the number of mismatches in HLA antigens between patients and their respective donors - for Class I - A and B (r = 0399, p <0.01) and for Class land Class II antigens - A, B and DR Cr = 0.409,p <0.01). No significant relationship was observed between the numbers of IFN-gamma-secreting cells and incidence of acute rejection (neither ACR, nor AMR). However, there was a trend of elevated frequencies of IFN-gamma-producing cells in patients who developed ACR or AMR in comparison with kidney recipients free of rejection (91 82 and 114 75 vs. 72 70/5 x 10(4) peripheral blood mononuclear cells respectively). Patients with concurrent acute cellular and antibody-mediated rejection had also higher numbers of IFN-y-producing memory/effector cells compared to patients with cellular rejection only. Conclusion: Pre-transplant determination of the numbers of IFN-y-producing donor-specific memory cells using the ELISpot technique may provide clinically relevant results when evaluating the risk of development of acute cellular and antibody-mediated rejection. These frequencies are influenced by the degree of HLA mismatching between patients and their respective kidney donors.

Název v anglickém jazyce

Pre-transplant donor-specific Interferon-gamma-producing cells and acute rejection of the kidney allograft

Popis výsledku anglicky

Background: Our retrospective study included a cohort of 47 patients who underwent living donor kidney transplantation. The pre-transplant frequencies of donor-specific Interferon-gamma (IFN-gamma) producing cells were defined using enzyme-linked immunosorbent spot (ELISpot) assay and correlated with incidence of acute cellular (ACR), antibody-mediated rejection (AMR) and kidney graft survival up to one year after transplantation. Results: We found a statistically significant correlation between the frequencies of IFN-gamma-producing cells and the number of mismatches in HLA antigens between patients and their respective donors - for Class I - A and B (r = 0399, p <0.01) and for Class land Class II antigens - A, B and DR Cr = 0.409,p <0.01). No significant relationship was observed between the numbers of IFN-gamma-secreting cells and incidence of acute rejection (neither ACR, nor AMR). However, there was a trend of elevated frequencies of IFN-gamma-producing cells in patients who developed ACR or AMR in comparison with kidney recipients free of rejection (91 82 and 114 75 vs. 72 70/5 x 10(4) peripheral blood mononuclear cells respectively). Patients with concurrent acute cellular and antibody-mediated rejection had also higher numbers of IFN-y-producing memory/effector cells compared to patients with cellular rejection only. Conclusion: Pre-transplant determination of the numbers of IFN-y-producing donor-specific memory cells using the ELISpot technique may provide clinically relevant results when evaluating the risk of development of acute cellular and antibody-mediated rejection. These frequencies are influenced by the degree of HLA mismatching between patients and their respective kidney donors.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/NT14022" target="_blank" >NT14022: DONOR-SPECIFICKÉ INTERFERON-GAMA PRODUKUJÍCÍ BUŇKY A PREDIKCE REJEKCE TRANSPLANTOVANÉ LEDVINY</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Transplant immunology

ISSN

0966-3274

e-ISSN

—

Svazek periodika

33

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

63-68

Kód UT WoS článku

000363818200003

EID výsledku v databázi Scopus

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