Pro-inflammatory gene expression in adipose tissue of patients with atherosclerosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F17%3A00076179" target="_blank" >RIV/00023001:_____/17:00076179 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Pro-inflammatory gene expression in adipose tissue of patients with atherosclerosis

Popis výsledku v původním jazyce

Inflammatory changes, both in the arterial wall and adipose tissue, play a crucial role in the development of atherosclerosis. We measured the gene expression of tumor necrosis factor-alpha (TNF alpha), monocyte chemoattractant protein-1 (MCP-1), and interleukin 6 (IL-6) in adipose tissue (AT) of living kidney donors (LKD) and patients with peripheral arterial disease (PAD). Quantitative polymerase chain reaction (qPCR) and flow cytometry analyses were performed in subcutaneous (SAT), visceral (VAT), and perivascular adipose tissue (PVAT). Data of PAD patients showed significantly higher expression in VAT in all three genes (TNFa 5-fold, p&lt;0.05; MCP-1 3.6-fold, p&lt;0.05; IL-6 18.8-fold, p&lt;0.001). The differences in PVAT and SAT were less significant. Total body pro-inflammatory status was documented by higher TNFa concentration in patients (4.86 +/- 1.4 pg/ml) compared to LKDs (2.14 +/- 0.9 pg/ml; p&lt;0.001), as was hsCRP (11.8 +/- 7.0 in PAD; 1.5 +/- 0.48 in LKDs; p=0.017). We found no age-dependent relationship between gene expression vs. TNFa and hsCRP concentrations in both compared groups. No effect of the atherosclerosis score on gene expression and circulating inflammatory markers within the PAD group was observed. Our results suggest that the AT of PAD patients infiltrated with macrophages produces more cytokines involved in the development of inflammation and atherosclerosis.

Název v anglickém jazyce

Pro-inflammatory gene expression in adipose tissue of patients with atherosclerosis

Popis výsledku anglicky

Inflammatory changes, both in the arterial wall and adipose tissue, play a crucial role in the development of atherosclerosis. We measured the gene expression of tumor necrosis factor-alpha (TNF alpha), monocyte chemoattractant protein-1 (MCP-1), and interleukin 6 (IL-6) in adipose tissue (AT) of living kidney donors (LKD) and patients with peripheral arterial disease (PAD). Quantitative polymerase chain reaction (qPCR) and flow cytometry analyses were performed in subcutaneous (SAT), visceral (VAT), and perivascular adipose tissue (PVAT). Data of PAD patients showed significantly higher expression in VAT in all three genes (TNFa 5-fold, p&lt;0.05; MCP-1 3.6-fold, p&lt;0.05; IL-6 18.8-fold, p&lt;0.001). The differences in PVAT and SAT were less significant. Total body pro-inflammatory status was documented by higher TNFa concentration in patients (4.86 +/- 1.4 pg/ml) compared to LKDs (2.14 +/- 0.9 pg/ml; p&lt;0.001), as was hsCRP (11.8 +/- 7.0 in PAD; 1.5 +/- 0.48 in LKDs; p=0.017). We found no age-dependent relationship between gene expression vs. TNFa and hsCRP concentrations in both compared groups. No effect of the atherosclerosis score on gene expression and circulating inflammatory markers within the PAD group was observed. Our results suggest that the AT of PAD patients infiltrated with macrophages produces more cytokines involved in the development of inflammation and atherosclerosis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological research

ISSN

0862-8408

e-ISSN

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Svazek periodika

66

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

633-640

Kód UT WoS článku

000412736000009

EID výsledku v databázi Scopus

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