Clinical and( 31)P magnetic resonance spectroscopy characterization of patients with critical limb ischemia before and after autologous cell therapy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00078318" target="_blank" >RIV/00023001:_____/19:00078318 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.biomed.cas.cz/physiolres/pdf/2019/68_559.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2019/68_559.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.934107" target="_blank" >10.33549/physiolres.934107</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Clinical and( 31)P magnetic resonance spectroscopy characterization of patients with critical limb ischemia before and after autologous cell therapy

Popis výsledku v původním jazyce

Autologous cell therapy (ACT) is a new treatment method for diabetic patients with critical limb ischemia (CLI) not eligible for standard revascularization. After intramuscular injection of bone marrow-derived mononuclear cells local arteriogenesis in the ischemic tissue occurs. Studies assessing visualization of this therapeutic vasculogenesis after ACT by novel imaging techniques are lacking. The aim of our study was to assess the effect of ACT on possible metabolic changes and perfusion of critically ischemic limbs using P- 31 magnetic resonance spectroscopy (P-31 MRS) and its possible correlation with changes of transcutaneous oxygen pressure (TcPO2). Twenty-one patients with diabetes and no-option CLI treated by ACT in our foot clinic over 8 years were included in the study. TcPO2 as well as rest (phosphocreatine, adenosine triphosphate and inorganic phosphate) and dynamic (mitochondria) capacity and phosphocreatine recovery time) P- 31-MRS parameters were evaluated at baseline and 3 months after cell treatment. TcPO2 increased significantly after 3 months compared with baseline (from 22.4 +/- 8.2 to 37.6 +/- 13.3 mm Hg, p=0.0002). Rest and dynamic P- 31 MRS parameters were not significantly different after ACT in comparison with baseline values. Our study showed a significant increase of TcPO2 on the dorsum of the foot after ACT. We did not observe any changes of rest or dynamic P- 31 MRS parameters in the area of the proximal calf where the cell suspension has been injected into.

Název v anglickém jazyce

Clinical and( 31)P magnetic resonance spectroscopy characterization of patients with critical limb ischemia before and after autologous cell therapy

Popis výsledku anglicky

Autologous cell therapy (ACT) is a new treatment method for diabetic patients with critical limb ischemia (CLI) not eligible for standard revascularization. After intramuscular injection of bone marrow-derived mononuclear cells local arteriogenesis in the ischemic tissue occurs. Studies assessing visualization of this therapeutic vasculogenesis after ACT by novel imaging techniques are lacking. The aim of our study was to assess the effect of ACT on possible metabolic changes and perfusion of critically ischemic limbs using P- 31 magnetic resonance spectroscopy (P-31 MRS) and its possible correlation with changes of transcutaneous oxygen pressure (TcPO2). Twenty-one patients with diabetes and no-option CLI treated by ACT in our foot clinic over 8 years were included in the study. TcPO2 as well as rest (phosphocreatine, adenosine triphosphate and inorganic phosphate) and dynamic (mitochondria) capacity and phosphocreatine recovery time) P- 31-MRS parameters were evaluated at baseline and 3 months after cell treatment. TcPO2 increased significantly after 3 months compared with baseline (from 22.4 +/- 8.2 to 37.6 +/- 13.3 mm Hg, p=0.0002). Rest and dynamic P- 31 MRS parameters were not significantly different after ACT in comparison with baseline values. Our study showed a significant increase of TcPO2 on the dorsum of the foot after ACT. We did not observe any changes of rest or dynamic P- 31 MRS parameters in the area of the proximal calf where the cell suspension has been injected into.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

<a href="/cs/project/NV16-27262A" target="_blank" >NV16-27262A: Srovnání vlivu aplikace autologních kmenových buněk a standardní revaskularizace na oxygenaci tkání a průběh syndromu diabetické nohy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological research

ISSN

0862-8408

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

559-566

Kód UT WoS článku

000483374200004

EID výsledku v databázi Scopus

2-s2.0-85071708639