The relationship of mitochondrial dysfunction and the development of insulin resistance in Cushing's syndrome
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00078342" target="_blank" >RIV/00023001:_____/19:00078342 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11110/19:10403093 RIV/00023728:_____/19:N0000078 RIV/00064165:_____/19:10403093
Výsledek na webu
<a href="https://www.dovepress.com/the-relationship-of-mitochondrial-dysfunction-and-the-development-of-i-peer-reviewed-fulltext-article-DMSO" target="_blank" >https://www.dovepress.com/the-relationship-of-mitochondrial-dysfunction-and-the-development-of-i-peer-reviewed-fulltext-article-DMSO</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2147/DMSO.S209095" target="_blank" >10.2147/DMSO.S209095</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The relationship of mitochondrial dysfunction and the development of insulin resistance in Cushing's syndrome
Popis výsledku v původním jazyce
Purpose: Cushing's syndrome is characterized by metabolic disturbances including insulin resistance. Mitochondrial dysfunction is one pathogenic factor in the development of insulin resistance in patients with obesity. We explored whether mitochondrial dysfunction correlates with insulin resistance and other metabolic complications. Patients and methods: We investigated the changes of mRNA expression of genes encoding selected subunits of oxidative phosphorylation system (OXPHOS), pyruvate dehydrogenase (PDH) and citrate synthase (CS) in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) and mitochondrial enzyme activity in platelets of 24 patients with active Cushing's syndrome and in 9 of them after successful treatment and 22 healthy control subjects. Results: Patients with active Cushing's syndrome had significantly increased body mass index (BMI), homeostasis model assessment of insulin resistance (HOMA-IR) and serum lipids relative to the control group. The expression of all investigated genes for selected mitochondrial proteins was decreased in SCAT in patients with active Cushing's syndrome and remained decreased after successful treatment. The expression of most tested genes in SCAT correlated inversely with BMI and HOMA-IR. The expression of genes encoding selected OXPHOS subunits and CS was increased in PM in patients with active Cushing's syndrome with a tendency to decrease toward normal levels after cure. Patients with active Cushing's syndrome showed increased enzyme activity of complex I (NQR) in platelets. Conclusion: Mitochondrial function in SCAT in patients with Cushing's syndrome is impaired and only slightly affected by its treatment which may reflect ongoing metabolic disturbances even after successful treatment of Cushing's syndrome.
Název v anglickém jazyce
The relationship of mitochondrial dysfunction and the development of insulin resistance in Cushing's syndrome
Popis výsledku anglicky
Purpose: Cushing's syndrome is characterized by metabolic disturbances including insulin resistance. Mitochondrial dysfunction is one pathogenic factor in the development of insulin resistance in patients with obesity. We explored whether mitochondrial dysfunction correlates with insulin resistance and other metabolic complications. Patients and methods: We investigated the changes of mRNA expression of genes encoding selected subunits of oxidative phosphorylation system (OXPHOS), pyruvate dehydrogenase (PDH) and citrate synthase (CS) in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) and mitochondrial enzyme activity in platelets of 24 patients with active Cushing's syndrome and in 9 of them after successful treatment and 22 healthy control subjects. Results: Patients with active Cushing's syndrome had significantly increased body mass index (BMI), homeostasis model assessment of insulin resistance (HOMA-IR) and serum lipids relative to the control group. The expression of all investigated genes for selected mitochondrial proteins was decreased in SCAT in patients with active Cushing's syndrome and remained decreased after successful treatment. The expression of most tested genes in SCAT correlated inversely with BMI and HOMA-IR. The expression of genes encoding selected OXPHOS subunits and CS was increased in PM in patients with active Cushing's syndrome with a tendency to decrease toward normal levels after cure. Patients with active Cushing's syndrome showed increased enzyme activity of complex I (NQR) in platelets. Conclusion: Mitochondrial function in SCAT in patients with Cushing's syndrome is impaired and only slightly affected by its treatment which may reflect ongoing metabolic disturbances even after successful treatment of Cushing's syndrome.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30202 - Endocrinology and metabolism (including diabetes, hormones)
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Diabetes, metabolic syndrome and obesity: Targets and therapy
ISSN
1178-7007
e-ISSN
—
Svazek periodika
12
Číslo periodika v rámci svazku
2019
Stát vydavatele periodika
NZ - Nový Zéland
Počet stran výsledku
13
Strana od-do
1459-1471
Kód UT WoS článku
000483444700001
EID výsledku v databázi Scopus
2-s2.0-85073344340