Early Changes during Skin Repair Using Tissue-Engineered Dermal Template in a Full-Thickness Burn

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F20%3A00081063" target="_blank" >RIV/00023001:_____/20:00081063 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/20:43921667

Výsledek na webu

<a href="https://fb.cuni.cz/file/5936/fb2020a0022.pdf" target="_blank" >https://fb.cuni.cz/file/5936/fb2020a0022.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Early Changes during Skin Repair Using Tissue-Engineered Dermal Template in a Full-Thickness Burn

Popis výsledku v původním jazyce

Rapid wound closure in extensively burned patients has remained one of the major unresolved issues of medicine. Integra (R) is the most widely established artificial skin, which is composed of a porous matrix of cross-linked bovine collagen and chondroitin 6-sulphate covered by a semi-permeable silicone layer. We present here a (immuno)histological study of a severely burned patient with a full-thickness burn treated with a tissue-engineered dermal template (Integra (R)) and split-thickness skin graft-based protocol. Immunohistochemical investigation of the artificial dermis revealed that immune cell infiltration reached its peak on day 10. Tissue immunophenotyping found an increase in CD3(+) cells over the course of the study as well as CD4 and CD8 positivity on day 40, indicating remaining T-cell subpopulations. We observed weak/no infiltration of NK cells (CD56(+)). In conclusion, the use of bi-layer Integra (R) represents a feasible and safe procedure resulting in formation of non-irritating dermal substitutes.

Název v anglickém jazyce

Early Changes during Skin Repair Using Tissue-Engineered Dermal Template in a Full-Thickness Burn

Popis výsledku anglicky

Rapid wound closure in extensively burned patients has remained one of the major unresolved issues of medicine. Integra (R) is the most widely established artificial skin, which is composed of a porous matrix of cross-linked bovine collagen and chondroitin 6-sulphate covered by a semi-permeable silicone layer. We present here a (immuno)histological study of a severely burned patient with a full-thickness burn treated with a tissue-engineered dermal template (Integra (R)) and split-thickness skin graft-based protocol. Immunohistochemical investigation of the artificial dermis revealed that immune cell infiltration reached its peak on day 10. Tissue immunophenotyping found an increase in CD3(+) cells over the course of the study as well as CD4 and CD8 positivity on day 40, indicating remaining T-cell subpopulations. We observed weak/no infiltration of NK cells (CD56(+)). In conclusion, the use of bi-layer Integra (R) represents a feasible and safe procedure resulting in formation of non-irritating dermal substitutes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30216 - Dermatology and venereal diseases

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia biologica (Praha)

ISSN

0015-5500

e-ISSN

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Svazek periodika

66

Číslo periodika v rámci svazku

5-6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

161-168

Kód UT WoS článku

000669523700002

EID výsledku v databázi Scopus

2-s2.0-85107792444