The effects of immunosuppressive drugs on the characteristics and functional properties of bone marrow-derived stem cells isolated from patients with diabetes mellitus and peripheral arterial disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F23%3A00084087" target="_blank" >RIV/00023001:_____/23:00084087 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10466412 RIV/00216208:11110/23:10466412

Výsledek na webu

<a href="https://www.mdpi.com/2227-9059/11/7/1872" target="_blank" >https://www.mdpi.com/2227-9059/11/7/1872</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/biomedicines11071872" target="_blank" >10.3390/biomedicines11071872</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effects of immunosuppressive drugs on the characteristics and functional properties of bone marrow-derived stem cells isolated from patients with diabetes mellitus and peripheral arterial disease

Popis výsledku v původním jazyce

Background: Diabetic patients (DPs) with foot ulcers can receive autologous cell therapy (ACT) as a last therapeutic option. Even DPs who have undergone organ transplantation and are using immunosuppressive (IS) drugs can be treated by ACT. The aim of our study was to analyze the effects of IS drugs on the characteristics of bone marrow-derived stem cells (BM-MSCs). Methods: The cells were isolated from the bone marrow of DPs, cultivated for 14-18 days, and phenotypically characterized using flow cytometry. These precursor cells were cultured in the presence of various IS drugs. The impact of IS drugs on metabolic activity was measured using a WST-1 assay, and the expression of genes for immunoregulatory molecules was detected through RT-PCR. Cell death was analyzed through the use of flow cytometry, and the production of cytokines was determined by ELISA. Results: The mononuclear fraction of cultured cells contained mesenchymal stem cells (CD45(-)CD73(+)CD90(+)CD105(+)), myeloid angiogenic cells (CD45(+)CD146(-)), and endothelial colony-forming cells (CD45(-)CD146(+)). IS drugs inhibited metabolic activity, the expression of genes for immunoregulatory molecules, the production of cytokines, and the viability of the cells. Conclusions: The results indicate that IS drugs in a dose-dependent manner had a negative impact on the properties of BM-MSCs used to treat ischemic diabetic foot ulcers, and that these drugs could affect the therapeutic potential of BM-MSCs.

Název v anglickém jazyce

The effects of immunosuppressive drugs on the characteristics and functional properties of bone marrow-derived stem cells isolated from patients with diabetes mellitus and peripheral arterial disease

Popis výsledku anglicky

Background: Diabetic patients (DPs) with foot ulcers can receive autologous cell therapy (ACT) as a last therapeutic option. Even DPs who have undergone organ transplantation and are using immunosuppressive (IS) drugs can be treated by ACT. The aim of our study was to analyze the effects of IS drugs on the characteristics of bone marrow-derived stem cells (BM-MSCs). Methods: The cells were isolated from the bone marrow of DPs, cultivated for 14-18 days, and phenotypically characterized using flow cytometry. These precursor cells were cultured in the presence of various IS drugs. The impact of IS drugs on metabolic activity was measured using a WST-1 assay, and the expression of genes for immunoregulatory molecules was detected through RT-PCR. Cell death was analyzed through the use of flow cytometry, and the production of cytokines was determined by ELISA. Results: The mononuclear fraction of cultured cells contained mesenchymal stem cells (CD45(-)CD73(+)CD90(+)CD105(+)), myeloid angiogenic cells (CD45(+)CD146(-)), and endothelial colony-forming cells (CD45(-)CD146(+)). IS drugs inhibited metabolic activity, the expression of genes for immunoregulatory molecules, the production of cytokines, and the viability of the cells. Conclusions: The results indicate that IS drugs in a dose-dependent manner had a negative impact on the properties of BM-MSCs used to treat ischemic diabetic foot ulcers, and that these drugs could affect the therapeutic potential of BM-MSCs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

<a href="/cs/project/LX22NPO5104" target="_blank" >LX22NPO5104: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedicines

ISSN

2227-9059

e-ISSN

2227-9059

Svazek periodika

11

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

"art. no. 1872"

Kód UT WoS článku

001037991500001

EID výsledku v databázi Scopus

2-s2.0-85175113930