Macrocomplexes: an establishing critical limits of post-PEG serum enzymes and proteins in adults and children

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F25%3A00085559" target="_blank" >RIV/00023001:_____/25:00085559 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/25:43928378

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0009898125002050?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0009898125002050?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cca.2025.120326" target="_blank" >10.1016/j.cca.2025.120326</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Macrocomplexes: an establishing critical limits of post-PEG serum enzymes and proteins in adults and children

Popis výsledku v původním jazyce

Background: Exclusion of serum macroform presence is essential to avoid potential misdiagnosis. The aim of this study was to determine critical limits (CL) for enzymes (total and pancreatic amylase, AMY and pAMY; aminotransferases, AST and ALT; alkaline phosphatase, ALP; creatine kinase, CK; lipase, LIP; gamma-glutamyl transferase, GGT; lactate dehydrogenase, LDH) after polyethylene glycol (PEG) precipitation in children and adults. Methods: A total of 126 sera of patients suspected for macroenzyme presence (adults or children) was matched by serum enzyme activity of controls and all these sera were precipitated by PEG. PEG-precipitable activity ( %PPA) and CLs of %PPA were calculated for adults, children, and controls. Results: CLs of %PPA were substantially lower than common 60 % for ALP and CK in all subjects. Significant differences between adults and children were found for ALT and ALP (lower in children), and LIP and CK (higher in children). No meaningful correlation was found between initial enzyme activity and %PPA. Conclusions: Age specific CLs should be used for ALP, ALT, CK, and LIP. Generally accepted value of %PPA of 60 % may be used with caution for AMY, pAMY, AST, GGT, and LDH. A careful selection of CLs is needed for timely detection of macrocomplexes.

Název v anglickém jazyce

Macrocomplexes: an establishing critical limits of post-PEG serum enzymes and proteins in adults and children

Popis výsledku anglicky

Background: Exclusion of serum macroform presence is essential to avoid potential misdiagnosis. The aim of this study was to determine critical limits (CL) for enzymes (total and pancreatic amylase, AMY and pAMY; aminotransferases, AST and ALT; alkaline phosphatase, ALP; creatine kinase, CK; lipase, LIP; gamma-glutamyl transferase, GGT; lactate dehydrogenase, LDH) after polyethylene glycol (PEG) precipitation in children and adults. Methods: A total of 126 sera of patients suspected for macroenzyme presence (adults or children) was matched by serum enzyme activity of controls and all these sera were precipitated by PEG. PEG-precipitable activity ( %PPA) and CLs of %PPA were calculated for adults, children, and controls. Results: CLs of %PPA were substantially lower than common 60 % for ALP and CK in all subjects. Significant differences between adults and children were found for ALT and ALP (lower in children), and LIP and CK (higher in children). No meaningful correlation was found between initial enzyme activity and %PPA. Conclusions: Age specific CLs should be used for ALP, ALT, CK, and LIP. Generally accepted value of %PPA of 60 % may be used with caution for AMY, pAMY, AST, GGT, and LDH. A careful selection of CLs is needed for timely detection of macrocomplexes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinica chimica acta

ISSN

0009-8981

e-ISSN

1873-3492

Svazek periodika

574

Číslo periodika v rámci svazku

15 June 2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

"art. no. 120326"

Kód UT WoS článku

001481468600001

EID výsledku v databázi Scopus

2-s2.0-105003238359