Watanabea acidotolerans: A new trebouxiophyte lineage (Chlorophyta) inhabiting low pH environments from Europe to South America

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023272%3A_____%2F19%3A10134641" target="_blank" >RIV/00023272:_____/19:10134641 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/19:10406989

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1111/pre.12357" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1111/pre.12357</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/pre.12357" target="_blank" >10.1111/pre.12357</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Watanabea acidotolerans: A new trebouxiophyte lineage (Chlorophyta) inhabiting low pH environments from Europe to South America

Popis výsledku v původním jazyce

Here we studied a new Watanabea strain CAUP H 8901-CRYO isolated from an extremely acidic (pH 2.6) pit lake in Czechia. We used morphological, ultrastructural and molecular data to ascertain its taxonomic position and phylogenetic relationships within the genus Watanabea. The morphology of the studied strain matched the formal Watanabea description, including its most characteristic feature, the production of two types of autospores. The new isolate morphologically and ultrastructurally resembled the Watanabea type species, W. reniformis. However, it differed from W. borysthenica by having a pyrenoid not surrounded by starch grains. Molecular phylogenetic analyses of nuclear 18S rDNA and plastid rbcL showed that the strain CAUP H 8901-CRYO formed an independent lineage within the genus Watanabea. Furthermore, analyses of nuclear ITS2 secondary structure demonstrated an additional side loop coiling from the helix III, the unique feature of CAUP H 8901-CRYO, not present in the authentic strain of W. reniformis SAG 211-9b. The ITS2 secondary structures of the two strains also differed by one compensatory base change (CBC) and a number of additional nucleotide substitutions. The new acidotolerant strain and another Watanabea isolate from a naturally acidic lake in Argentina were identical in their plastid rbcL sequence. Consequently, here we proposed a new species, Watanabea acidotolerans, to accommodate a lineage of microalgae with a possible worldwide distribution and tolerance to low pH habitats.

Název v anglickém jazyce

Watanabea acidotolerans: A new trebouxiophyte lineage (Chlorophyta) inhabiting low pH environments from Europe to South America

Popis výsledku anglicky

Here we studied a new Watanabea strain CAUP H 8901-CRYO isolated from an extremely acidic (pH 2.6) pit lake in Czechia. We used morphological, ultrastructural and molecular data to ascertain its taxonomic position and phylogenetic relationships within the genus Watanabea. The morphology of the studied strain matched the formal Watanabea description, including its most characteristic feature, the production of two types of autospores. The new isolate morphologically and ultrastructurally resembled the Watanabea type species, W. reniformis. However, it differed from W. borysthenica by having a pyrenoid not surrounded by starch grains. Molecular phylogenetic analyses of nuclear 18S rDNA and plastid rbcL showed that the strain CAUP H 8901-CRYO formed an independent lineage within the genus Watanabea. Furthermore, analyses of nuclear ITS2 secondary structure demonstrated an additional side loop coiling from the helix III, the unique feature of CAUP H 8901-CRYO, not present in the authentic strain of W. reniformis SAG 211-9b. The ITS2 secondary structures of the two strains also differed by one compensatory base change (CBC) and a number of additional nucleotide substitutions. The new acidotolerant strain and another Watanabea isolate from a naturally acidic lake in Argentina were identical in their plastid rbcL sequence. Consequently, here we proposed a new species, Watanabea acidotolerans, to accommodate a lineage of microalgae with a possible worldwide distribution and tolerance to low pH habitats.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Phycological Research

ISSN

1322-0829

e-ISSN

—

Svazek periodika

67

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

8

Strana od-do

120-127

Kód UT WoS článku

000462903300005

EID výsledku v databázi Scopus

2-s2.0-85058092734