Medically assisted reproduction significantly affects the expression of microRNA associated with adverse obstetric outcomes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023698%3A_____%2F25%3AN0000008" target="_blank" >RIV/00023698:_____/25:N0000008 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/25:43928999

Výsledek na webu

<a href="https://doi.org/10.1016/j.rbmo.2025.105130" target="_blank" >https://doi.org/10.1016/j.rbmo.2025.105130</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.rbmo.2025.105130" target="_blank" >10.1016/j.rbmo.2025.105130</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Medically assisted reproduction significantly affects the expression of microRNA associated with adverse obstetric outcomes

Popis výsledku v původním jazyce

Research question: Do normal term pregnancies conceived by medically assisted reproduction (MAR) differ in the expression of microRNA associated with adverse obstetric outcomes compared with normal term pregnancies after natural conception, and are there differences in microRNA expression profiles between MAR groups? Design: This retrospective case-control study examined 103 pregnancies conceived by MAR and 79 pregnancies conceived naturally. The expression of 29 microRNA associated with adverse obstetric outcomes was evaluated in maternal peripheral blood leukocytes collected at 10-13 weeks of gestation. Results: Pregnancies after MAR showed significant changes in expression of 4-9 microRNA depending on the specific MAR group (IVF/intracytoplasmic sperm injection [ICSI] fresh embryo transfer group, 9 microRNA; intrauterine insemination group, 6 microRNA; IVF/ICSI frozen embryo transfer group, 4 microRNA) compared with pregnancies after natural conception in the early stages of pregnancy. Remarkably, some of these changes overlapped with those found in the early stages of pregnancies with a subsequent occurrence of pre-eclampsia, HELLP syndrome, fetal growth restriction, small for gestational age, gestational diabetes, preterm delivery without other pregnancy complications, miscarriage or stillbirth. Conclusions: This study is the first to show that even normal term pregnancies after MAR show significantly altered microRNA expression profiles in the first trimester of gestation compared with pregnancies after natural conception. The degree of dysregulation in microRNA expression varied depending on the MAR used, and the observed changes were partially consistent with those found in pregnancies that later developed pregnancy-related complications. These findings suggest that MAR significantly affects the epigenetic profile.

Název v anglickém jazyce

Medically assisted reproduction significantly affects the expression of microRNA associated with adverse obstetric outcomes

Popis výsledku anglicky

Research question: Do normal term pregnancies conceived by medically assisted reproduction (MAR) differ in the expression of microRNA associated with adverse obstetric outcomes compared with normal term pregnancies after natural conception, and are there differences in microRNA expression profiles between MAR groups? Design: This retrospective case-control study examined 103 pregnancies conceived by MAR and 79 pregnancies conceived naturally. The expression of 29 microRNA associated with adverse obstetric outcomes was evaluated in maternal peripheral blood leukocytes collected at 10-13 weeks of gestation. Results: Pregnancies after MAR showed significant changes in expression of 4-9 microRNA depending on the specific MAR group (IVF/intracytoplasmic sperm injection [ICSI] fresh embryo transfer group, 9 microRNA; intrauterine insemination group, 6 microRNA; IVF/ICSI frozen embryo transfer group, 4 microRNA) compared with pregnancies after natural conception in the early stages of pregnancy. Remarkably, some of these changes overlapped with those found in the early stages of pregnancies with a subsequent occurrence of pre-eclampsia, HELLP syndrome, fetal growth restriction, small for gestational age, gestational diabetes, preterm delivery without other pregnancy complications, miscarriage or stillbirth. Conclusions: This study is the first to show that even normal term pregnancies after MAR show significantly altered microRNA expression profiles in the first trimester of gestation compared with pregnancies after natural conception. The degree of dysregulation in microRNA expression varied depending on the MAR used, and the observed changes were partially consistent with those found in pregnancies that later developed pregnancy-related complications. These findings suggest that MAR significantly affects the epigenetic profile.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30214 - Obstetrics and gynaecology

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

REPRODUCTIVE BIOMEDICINE ONLINE

ISSN

1472-6483

e-ISSN

1472-6491

Svazek periodika

51

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

105130

Kód UT WoS článku

001610701700001

EID výsledku v databázi Scopus

2-s2.0-105020093404