Muscle-specific miRNAs in plasma and skeletal muscle of patients with idiopathic inflammatory myopathy are modulated by disease and training.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023728%3A_____%2F25%3AN0000081" target="_blank" >RIV/00023728:_____/25:N0000081 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/25:00385294 RIV/00023728:_____/25:N0000007

Výsledek na webu

<a href="https://doi.org/10.1093/rheumatology/keae704" target="_blank" >https://doi.org/10.1093/rheumatology/keae704</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/rheumatology/keae704" target="_blank" >10.1093/rheumatology/keae704</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Muscle-specific miRNAs in plasma and skeletal muscle of patients with idiopathic inflammatory myopathy are modulated by disease and training.

Popis výsledku v původním jazyce

The objective of this work was to examine myomiR levels in plasma, skeletal muscle, and skeletal muscle cells of patients with idiopathic inflammatory myopathy (IIM), and their interrelations with the disease-related clinical phenotypes and with the effects of a disease-modifying 6-months-training intervention. Samples of vastus lateralis muscle (n = 12/13) and plasma (n = 21/20) were obtained from IIM patients and healthy controls, respectively. The muscle and plasma samples were obtained before and after a 6-months training intervention in 7 patients. MyomiRs miR-1, -206, -133a, and -133b were quantified using quantitative PCR (qPCR). The effects of pro-inflammatory (TNF) and metabolic (glucose, insulin) systemic factors and of immunosuppressive therapy (dexamethasone) on the myomiRs were examined in muscle cells in vitro. MiR-133b was lower in the skeletal muscle of IIM patients than in that of healthy controls (P = 0.03). The levels of miR-133a, miR-1, and miR-206 were not regulated. Moreover, the plasma levels of miR-133b and miR-1 were reduced in patients with IIM compared with those in healthy controls (P <0.05). It was observed that exercise induced reciprocal regulation of specific myomiRs in the muscle and plasma of patients with IIM: it lowered miR-133b in muscle while increasing miR-133b and miR-206 in plasma. Treatment of myotubes with TNF, insulin, glucose and dexamethasone (individually) induced the downregulation of distinct myomiRs. Lower myomiR levels in the skeletal muscle of IIM patients might indicate reduced muscle regenerative potential in IIM, which could be linked to inflammation, metabolic dysfunction, and immunosuppressive therapy. Training-induced changes in muscle and plasma myomiRs indicate an increase in the release of myomiRs, which could contribute to the adaptive response underlying the positive systemic effects of exercise in IIM.

Název v anglickém jazyce

Muscle-specific miRNAs in plasma and skeletal muscle of patients with idiopathic inflammatory myopathy are modulated by disease and training.

Popis výsledku anglicky

The objective of this work was to examine myomiR levels in plasma, skeletal muscle, and skeletal muscle cells of patients with idiopathic inflammatory myopathy (IIM), and their interrelations with the disease-related clinical phenotypes and with the effects of a disease-modifying 6-months-training intervention. Samples of vastus lateralis muscle (n = 12/13) and plasma (n = 21/20) were obtained from IIM patients and healthy controls, respectively. The muscle and plasma samples were obtained before and after a 6-months training intervention in 7 patients. MyomiRs miR-1, -206, -133a, and -133b were quantified using quantitative PCR (qPCR). The effects of pro-inflammatory (TNF) and metabolic (glucose, insulin) systemic factors and of immunosuppressive therapy (dexamethasone) on the myomiRs were examined in muscle cells in vitro. MiR-133b was lower in the skeletal muscle of IIM patients than in that of healthy controls (P = 0.03). The levels of miR-133a, miR-1, and miR-206 were not regulated. Moreover, the plasma levels of miR-133b and miR-1 were reduced in patients with IIM compared with those in healthy controls (P <0.05). It was observed that exercise induced reciprocal regulation of specific myomiRs in the muscle and plasma of patients with IIM: it lowered miR-133b in muscle while increasing miR-133b and miR-206 in plasma. Treatment of myotubes with TNF, insulin, glucose and dexamethasone (individually) induced the downregulation of distinct myomiRs. Lower myomiR levels in the skeletal muscle of IIM patients might indicate reduced muscle regenerative potential in IIM, which could be linked to inflammation, metabolic dysfunction, and immunosuppressive therapy. Training-induced changes in muscle and plasma myomiRs indicate an increase in the release of myomiRs, which could contribute to the adaptive response underlying the positive systemic effects of exercise in IIM.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30226 - Rheumatology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RHEUMATOLOGY

ISSN

1462-0324

e-ISSN

1462-0332

Svazek periodika

64

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

4347-4356

Kód UT WoS článku

001497875800001

EID výsledku v databázi Scopus

2-s2.0-105009971060