Monoclonal antibody to human chronic myeloid leukemia cell line MOLM-7 specifically reacts with an antigen of apoptotic cells.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F02%3A00000070" target="_blank" >RIV/00023736:_____/02:00000070 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Monoclonal antibody to human chronic myeloid leukemia cell line MOLM-7 specifically reacts with an antigen of apoptotic cells.

Popis výsledku v původním jazyce

Monoclonal antibody 2E12 was prepared by immunization of mice with fresh cells of chronic myeloid leukemia cell line MOLM-7. Subset of cells of 15 tested cell lines and 10 cultured patients leukemic cells was positive with the 2E12 antibody. KG-1 and HL-60 cell lines were treated by camptothecin for 4 hours, washed and further cultivated up 48 hours. A significant increase of the positivity with 2E12 was detected after 24 and 48 hours of culture of both cell lines, which well correlated with the increase of mitochondial apoptotic antigen Apo 2.7 and with the subG1 peaks. The 2E12 positive cells were morphologically in the programmmed cell death, possibly apoptosis. It is suggested, that 2E12 antibody detects an antigen on apoptotic cells, which can bepart of the signaling process for the ingestion by phagocytes.

Název v anglickém jazyce

Monoclonal antibody to human chronic myeloid leukemia cell line MOLM-7 specifically reacts with an antigen of apoptotic cells.

Popis výsledku anglicky

Monoclonal antibody 2E12 was prepared by immunization of mice with fresh cells of chronic myeloid leukemia cell line MOLM-7. Subset of cells of 15 tested cell lines and 10 cultured patients leukemic cells was positive with the 2E12 antibody. KG-1 and HL-60 cell lines were treated by camptothecin for 4 hours, washed and further cultivated up 48 hours. A significant increase of the positivity with 2E12 was detected after 24 and 48 hours of culture of both cell lines, which well correlated with the increase of mitochondial apoptotic antigen Apo 2.7 and with the subG1 peaks. The 2E12 positive cells were morphologically in the programmmed cell death, possibly apoptosis. It is suggested, that 2E12 antibody detects an antigen on apoptotic cells, which can bepart of the signaling process for the ingestion by phagocytes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Leukemia Research

ISSN

0145-2126

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

45-54

Kód UT WoS článku

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EID výsledku v databázi Scopus

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