Interaction of P-selectin and PSGL-1 generates microparticles that correct hemostasis in a mouse model of hemophilia A.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F03%3A00000148" target="_blank" >RIV/00023736:_____/03:00000148 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of P-selectin and PSGL-1 generates microparticles that correct hemostasis in a mouse model of hemophilia A.

Popis výsledku v původním jazyce

High plasma levels of soluble P-selectin are associated with thrombotic disorders and may predict future cardiovascular events. Mice with high levels of soluble P-selectin have increased numbers of microparticles in plasma. We now show in human blood that P-selectin-immunoglobulin chimeras (P-sel-Ig) induced formation of procoagulant microparticles through P-selectin glycoprotein ligand-1 (PSGL-1). In addition, Psgl-1-/- mice produced fewer microparticles after P-sel-Ig infusion and did not spontaneously increase their microparticles count in old age as do wild-type mice. Injected microparticles specifically bound to thrombi and thus could be involved in thrombin generation at sites of injury. Infusion of P-sel-Ig into hemophilia A mice produced a twenty-fold increase over control immunoglobulin in microparticles containing tissue factor. This significantly improved the kinetics of fibrin formation in the hemophilia A mice and normalized their tail-bleeding time.

Název v anglickém jazyce

Interaction of P-selectin and PSGL-1 generates microparticles that correct hemostasis in a mouse model of hemophilia A.

Popis výsledku anglicky

High plasma levels of soluble P-selectin are associated with thrombotic disorders and may predict future cardiovascular events. Mice with high levels of soluble P-selectin have increased numbers of microparticles in plasma. We now show in human blood that P-selectin-immunoglobulin chimeras (P-sel-Ig) induced formation of procoagulant microparticles through P-selectin glycoprotein ligand-1 (PSGL-1). In addition, Psgl-1-/- mice produced fewer microparticles after P-sel-Ig infusion and did not spontaneously increase their microparticles count in old age as do wild-type mice. Injected microparticles specifically bound to thrombi and thus could be involved in thrombin generation at sites of injury. Infusion of P-sel-Ig into hemophilia A mice produced a twenty-fold increase over control immunoglobulin in microparticles containing tissue factor. This significantly improved the kinetics of fibrin formation in the hemophilia A mice and normalized their tail-bleeding time.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

<a href="/cs/project/NM7719" target="_blank" >NM7719: Molekulárně-biologická diagnostika trombotické trombocytopenické purpury.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Medicine

ISSN

1078-8956

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

1020-1025

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—