Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F16%3A00011564" target="_blank" >RIV/00023736:_____/16:00011564 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/16:00463754

Výsledek na webu

<a href="http://dx.doi.org/10.1039/C6AY01833E" target="_blank" >http://dx.doi.org/10.1039/C6AY01833E</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/C6AY01833E" target="_blank" >10.1039/C6AY01833E</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

Popis výsledku v původním jazyce

Nonspecific protein adsorption, referred to as fouling, is a major clinical problem accompanying any material coming into direct contact with biological fluids, especially blood/plasma. Not only is protein fouling detrimental per se but it also promotes platelet adhesion, the initiation of blood coagulation, and/or complement activation. In spite of efforts to develop novel surfaces which suppress protein fouling, still only little is known about the mechanisms behind protein fouling from blood and other complex biological media. In order to unravel the mechanisms, precise identification of the proteins that foul the surface is necessary. This is particularly challenging for those surfaces with reduced protein fouling, such as polymer brushes. This report introduces a route to analyze the proteins by mass spectrometry using an optimized protocol. We demonstrate proteomics-compatible buffers/solutions suitable for desorption of adsorbed proteins on low fouling polymer brushes.

Název v anglickém jazyce

Total removal of intact blood plasma proteins deposited on surface-grafted polymer brushes

Popis výsledku anglicky

Nonspecific protein adsorption, referred to as fouling, is a major clinical problem accompanying any material coming into direct contact with biological fluids, especially blood/plasma. Not only is protein fouling detrimental per se but it also promotes platelet adhesion, the initiation of blood coagulation, and/or complement activation. In spite of efforts to develop novel surfaces which suppress protein fouling, still only little is known about the mechanisms behind protein fouling from blood and other complex biological media. In order to unravel the mechanisms, precise identification of the proteins that foul the surface is necessary. This is particularly challenging for those surfaces with reduced protein fouling, such as polymer brushes. This report introduces a route to analyze the proteins by mass spectrometry using an optimized protocol. We demonstrate proteomics-compatible buffers/solutions suitable for desorption of adsorbed proteins on low fouling polymer brushes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical methods

ISSN

1759-9660

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

6415-6419

Kód UT WoS článku

000382118500005

EID výsledku v databázi Scopus

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