In vivo validation of alternative FDXR transcripts in human blood in response to ionizing radiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F20%3A00013136" target="_blank" >RIV/00023736:_____/20:00013136 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00179906:_____/20:10419282

Výsledek na webu

<a href="https://doi.org/10.3390/ijms21217851" target="_blank" >https://doi.org/10.3390/ijms21217851</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21217851" target="_blank" >10.3390/ijms21217851</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vivo validation of alternative FDXR transcripts in human blood in response to ionizing radiation

Popis výsledku v původním jazyce

Following cell stress such as ionising radiation (IR) exposure, multiple cellular pathways are activated. We recently demonstrated that ferredoxin reductase (FDXR) has a remarkable IR-induced transcriptional responsiveness in blood. Here, we provided a first comprehensive FDXR variant profile following DNA damage. First, specific quantitative real-time polymerase chain reaction (qPCR) primers were designed to establish dose-responses for eight curated FDXR variants, all up-regulated after IR in a dose-dependent manner. The potential role of gender on the expression of these variants was tested, and neither the variants response to IR nor the background level of expression was profoundly affected, moreover, in vitro induction of inflammation temporarily counteracted IR response early after exposure.

Název v anglickém jazyce

In vivo validation of alternative FDXR transcripts in human blood in response to ionizing radiation

Popis výsledku anglicky

Following cell stress such as ionising radiation (IR) exposure, multiple cellular pathways are activated. We recently demonstrated that ferredoxin reductase (FDXR) has a remarkable IR-induced transcriptional responsiveness in blood. Here, we provided a first comprehensive FDXR variant profile following DNA damage. First, specific quantitative real-time polymerase chain reaction (qPCR) primers were designed to establish dose-responses for eight curated FDXR variants, all up-regulated after IR in a dose-dependent manner. The potential role of gender on the expression of these variants was tested, and neither the variants response to IR nor the background level of expression was profoundly affected, moreover, in vitro induction of inflammation temporarily counteracted IR response early after exposure.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30205 - Hematology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International journal of molecular sciences

ISSN

1422-0067

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

"art. no. 7851"

Kód UT WoS článku

000589122100001

EID výsledku v databázi Scopus

2-s2.0-85094122103