Age- and sex-dependent effects of ethanol on hippocampal hemicholinium-3 sensitive choline carriers during postnatal development of rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F03%3A00000305" target="_blank" >RIV/00023752:_____/03:00000305 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Age- and sex-dependent effects of ethanol on hippocampal hemicholinium-3 sensitive choline carriers during postnatal development of rats

Popis výsledku v původním jazyce

Vulnerability of hippocampal hemicholinium-3 (HC-3) sensitive carriers to ethanol was evaluated in vitro during rat postnatal development. The high-affinity uptake of (3H)choline (HACU) and the specific binding of (3H)HC-3 were measured on synaptosomes from 7-, 14-, 60-day and 3-month old male and female Wistar rats. Marked increases of basal (between 7 and 60 days of age) and of stimulated HACU levels via K+-depolarization (between 14 days and 3 months) but only a mild elevation in (3H)HC-3 binding (between 7 days and 3 months) associated with alterations in the binding site number were found. On the mature tissue, ethanol at high concentrations (5%) moderately inhibited the choline transport under basal conditions but totally eliminated depolarization effects. However, both age- and sex-dependent alterations in basal HACU mediated by high or low pharmacologically relevant alcohol concentrations (50-100 mM) were observed in the immature tissue. Namely, the dose- and incubation time-de

Název v anglickém jazyce

Age- and sex-dependent effects of ethanol on hippocampal hemicholinium-3 sensitive choline carriers during postnatal development of rats

Popis výsledku anglicky

Vulnerability of hippocampal hemicholinium-3 (HC-3) sensitive carriers to ethanol was evaluated in vitro during rat postnatal development. The high-affinity uptake of (3H)choline (HACU) and the specific binding of (3H)HC-3 were measured on synaptosomes from 7-, 14-, 60-day and 3-month old male and female Wistar rats. Marked increases of basal (between 7 and 60 days of age) and of stimulated HACU levels via K+-depolarization (between 14 days and 3 months) but only a mild elevation in (3H)HC-3 binding (between 7 days and 3 months) associated with alterations in the binding site number were found. On the mature tissue, ethanol at high concentrations (5%) moderately inhibited the choline transport under basal conditions but totally eliminated depolarization effects. However, both age- and sex-dependent alterations in basal HACU mediated by high or low pharmacologically relevant alcohol concentrations (50-100 mM) were observed in the immature tissue. Namely, the dose- and incubation time-de

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neurochemical Research

ISSN

0364-3190

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

3/4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

397-405

Kód UT WoS článku

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EID výsledku v databázi Scopus

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