Simultánní determinace dehydroepiandrosteronu, jeho hyxydroxylovaných metabolitů a jejich sulfátů v mozkové tkáni potkana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F04%3A00000387" target="_blank" >RIV/00023752:_____/04:00000387 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023761:_____/04:00011437

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous determination of dehydroepiandrosterone, its 7-hydroxylated metabolites, and their sulfates in rat brain tissues

Popis výsledku v původním jazyce

A method is described for simultaneous assesment of dehydroepiandrosterone (DHEA), its sulfate (DHEAS), and their 7-hydroxylated metabolites in cortex and subcortex of the rat brain. The procedure for determination of unconjugated steroids and DHEAS involved diethyl ether extraction of the homogenized tissue, solvent partition of the dry extract, and final quantification ba specific radioimmunoassays. In addition, determination of 7-hydroxydehydroepiandrosterone sufates required solvolysis, followed byhigh-performance liguid chromatography for separation of 7-hydroxylated metabolites from their precursor. The losses during this process were monitored by measurement of spiked radioactivity of ( (3)H)testosterone or ((3)H) dehydroepiandosterone sulfate.The content of dehydroepiandrosterone sulfate in both brain tissues was of the order of ten(s) nmol/g tissue irrecpective its type (cortex or subcortex), while concentrations of other steroids were about 10 times lower in both tissues. I

Název v anglickém jazyce

Simultaneous determination of dehydroepiandrosterone, its 7-hydroxylated metabolites, and their sulfates in rat brain tissues

Popis výsledku anglicky

A method is described for simultaneous assesment of dehydroepiandrosterone (DHEA), its sulfate (DHEAS), and their 7-hydroxylated metabolites in cortex and subcortex of the rat brain. The procedure for determination of unconjugated steroids and DHEAS involved diethyl ether extraction of the homogenized tissue, solvent partition of the dry extract, and final quantification ba specific radioimmunoassays. In addition, determination of 7-hydroxydehydroepiandrosterone sufates required solvolysis, followed byhigh-performance liguid chromatography for separation of 7-hydroxylated metabolites from their precursor. The losses during this process were monitored by measurement of spiked radioactivity of ( (3)H)testosterone or ((3)H) dehydroepiandosterone sulfate.The content of dehydroepiandrosterone sulfate in both brain tissues was of the order of ten(s) nmol/g tissue irrecpective its type (cortex or subcortex), while concentrations of other steroids were about 10 times lower in both tissues. I

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Steroids

ISSN

0039 - 128X

e-ISSN

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Svazek periodika

69

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

667-674

Kód UT WoS článku

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EID výsledku v databázi Scopus

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