Stat3 Silencing Affects Circadian Clock Gene Expression and Lipopolysaccharide Response in the Suprachiasmatic Nucleus, Cortex, and Glioblastoma Cell Cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F25%3A43921512" target="_blank" >RIV/00023752:_____/25:43921512 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/25:43928413 RIV/00216208:11310/25:10497865

Výsledek na webu

<a href="https://doi.org/10.1096/fj.202403177RR" target="_blank" >https://doi.org/10.1096/fj.202403177RR</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1096/fj.202403177RR" target="_blank" >10.1096/fj.202403177RR</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stat3 Silencing Affects Circadian Clock Gene Expression and Lipopolysaccharide Response in the Suprachiasmatic Nucleus, Cortex, and Glioblastoma Cell Cultures

Popis výsledku v původním jazyce

In mammals, the suprachiasmatic nucleus (SCN) serves as the central circadian pacemaker, regulating rhythms essential for physiological processes. STAT3, a transcription factor primarily involved in immune signaling, exhibits circadian rhythmicity in SCN astrocytes. This study examined the role of STAT3 in circadian regulation across several cell types, including primary cultures of rat SCN and cortex, SCN cells and organotypic SCN slices from PER2::LUC mice, and C6 glioblastoma cells. Furthermore, the involvement of STAT3 in inflammatory responses was investigated in SCN and cortical primary cultures. STAT3 silencing enhanced Bmal1 expression across all tested cell types, disrupted Bmal1 rhythmicity in C6 cells, and reduced the amplitude of the PER2-driven rhythm in bioluminescence in SCN primary cells and organotypic cultures. In SCN cells, STAT3 silencing also attenuated its own expression and Gfap, whereas in cortical cells, it exhibited broader effects. Under LPS stimulation, STAT3 silencing in SCN cells reduced most LPS-induced genes, including inflammatory and oxidative stress markers, while showing variable effects in cortical cells. These findings indicate that while the role of STAT3 in the circadian clockwork appears consistent across cell types, its involvement in functional gene expression and immune responses may vary depending on the tissue and differ between SCN and cortical primary cells.

Název v anglickém jazyce

Stat3 Silencing Affects Circadian Clock Gene Expression and Lipopolysaccharide Response in the Suprachiasmatic Nucleus, Cortex, and Glioblastoma Cell Cultures

Popis výsledku anglicky

In mammals, the suprachiasmatic nucleus (SCN) serves as the central circadian pacemaker, regulating rhythms essential for physiological processes. STAT3, a transcription factor primarily involved in immune signaling, exhibits circadian rhythmicity in SCN astrocytes. This study examined the role of STAT3 in circadian regulation across several cell types, including primary cultures of rat SCN and cortex, SCN cells and organotypic SCN slices from PER2::LUC mice, and C6 glioblastoma cells. Furthermore, the involvement of STAT3 in inflammatory responses was investigated in SCN and cortical primary cultures. STAT3 silencing enhanced Bmal1 expression across all tested cell types, disrupted Bmal1 rhythmicity in C6 cells, and reduced the amplitude of the PER2-driven rhythm in bioluminescence in SCN primary cells and organotypic cultures. In SCN cells, STAT3 silencing also attenuated its own expression and Gfap, whereas in cortical cells, it exhibited broader effects. Under LPS stimulation, STAT3 silencing in SCN cells reduced most LPS-induced genes, including inflammatory and oxidative stress markers, while showing variable effects in cortical cells. These findings indicate that while the role of STAT3 in the circadian clockwork appears consistent across cell types, its involvement in functional gene expression and immune responses may vary depending on the tissue and differ between SCN and cortical primary cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/LX22NPO5107" target="_blank" >LX22NPO5107: Národní ústav pro neurologický výzkum</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The FASEB Journal

ISSN

0892-6638

e-ISSN

1530-6860

Svazek periodika

39

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

"Article number e70577"

Kód UT WoS článku

001487870800001

EID výsledku v databázi Scopus

2-s2.0-105005219702