Sekvenční analýza 5'- části GLU-D1 pšenice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F00%3A107" target="_blank" >RIV/00027006:_____/00:107 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum)

Popis výsledku v původním jazyce

We have analyzed 380bp long N-terminal parts of Glu-D1x alleles from several lines of landraces and old cultivars of wheat (Triticum aestivum L.) with the aim to verify, whether alleles characterized as identical according to their mobility in SDS-PAGE are encoded always by the same DNA sequences. European landraces and old cultivars were chosen for the study as a potential source of higher genetic variability as compared with modern wheat cultivars. We have not found any changes in comparison with known data in the sequences of Dx3 and Dx4 alleles, which in fact were rare in the available set of landraces and old cultivars. Three point mutations were found together in Dx2 and Dx5 alleles of landraces and old cultivars, no one in the standard wheat cultivars Chinese Spring and Bezostaya1. Sequencing analysis can be another suitable technique for study of glutenin alleles complementing SDS-PAGE.

Název v anglickém jazyce

Sequence analysis of the 5'-terminal part of GLU-D1 locus of landraces and old cultivars of wheat (Triticum aestivum)

Popis výsledku anglicky

We have analyzed 380bp long N-terminal parts of Glu-D1x alleles from several lines of landraces and old cultivars of wheat (Triticum aestivum L.) with the aim to verify, whether alleles characterized as identical according to their mobility in SDS-PAGE are encoded always by the same DNA sequences. European landraces and old cultivars were chosen for the study as a potential source of higher genetic variability as compared with modern wheat cultivars. We have not found any changes in comparison with known data in the sequences of Dx3 and Dx4 alleles, which in fact were rare in the available set of landraces and old cultivars. Three point mutations were found together in Dx2 and Dx5 alleles of landraces and old cultivars, no one in the standard wheat cultivars Chinese Spring and Bezostaya1. Sequencing analysis can be another suitable technique for study of glutenin alleles complementing SDS-PAGE.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/GA521%2F97%2F0222" target="_blank" >GA521/97/0222: Molekulární analýza gliadinových a gluteninových genů u pšenice a příbuzných druhů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cereal Research Communications

ISSN

0133-3720

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

HU - Maďarsko

Počet stran výsledku

3

Strana od-do

414-416

Kód UT WoS článku

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EID výsledku v databázi Scopus

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