AFLP založené na fluorescenci je nejvhodnějším markerovacím systémem pro identifikaci odrůd řepky

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23%2383" target="_blank" >RIV/00027006:_____/04:######83 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/04:83

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification

Popis výsledku v původním jazyce

Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showedlhal the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism.Five microsatellites out of fifteen tested were polymorphic, but in a111oci, except one, on1y two different alleles were detected. This resu1t indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinationsdetected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence

Název v anglickém jazyce

Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification

Popis výsledku anglicky

Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showedlhal the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism.Five microsatellites out of fifteen tested were polymorphic, but in a111oci, except one, on1y two different alleles were detected. This resu1t indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinationsdetected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Genetics

ISSN

1234-1983

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

161-173

Kód UT WoS článku

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EID výsledku v databázi Scopus

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