Ověření STS márkerů genů odolnosti proti rzi u pšenice sedmi evropskými laboratořemi

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A94" target="_blank" >RIV/00027006:_____/04:94 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Verification of STS markers for leaf rust resistance genes of wheat by seven European laboratories

Popis výsledku v původním jazyce

A set of Thatcher near-isogenic lines and two breeding lines were used to examine sequence tagged site (STS) markers linked to leaf rust resistance genes Lr9, LrlO, Lr19, Lr24, Lr28, Lr29, Lr35, and a simple sequenced repeat (SSR) marker for Lr39. The selected STS markers for resistance genes Lr9, LrlO, Lr19, Lr24 and Lr28 were identified in seven accessions by seven European laboratories. Near-isogenic lines of the spring wheat Thatcher were used as positive controls. Markers for resistance genes Lr9,LrlO, Lr19, Lr24 were identified in all seven laboratories as amplification products of 1100 bp, 310 bp, 130bp and 310 bp, respectively. The STS markers linked to resistance genes Lr9, LrlO, Lr19, Lr24, Lr29, Lr35 and the SSR marker for Lr39 were robustand highly specific for these genes and will be useful in marker-assisted selection in wheat. However, the amplification product of 378 bp that corresponded with resistance gene Lr28 was detected in all accessions including genotypes lack

Název v anglickém jazyce

Verification of STS markers for leaf rust resistance genes of wheat by seven European laboratories

Popis výsledku anglicky

A set of Thatcher near-isogenic lines and two breeding lines were used to examine sequence tagged site (STS) markers linked to leaf rust resistance genes Lr9, LrlO, Lr19, Lr24, Lr28, Lr29, Lr35, and a simple sequenced repeat (SSR) marker for Lr39. The selected STS markers for resistance genes Lr9, LrlO, Lr19, Lr24 and Lr28 were identified in seven accessions by seven European laboratories. Near-isogenic lines of the spring wheat Thatcher were used as positive controls. Markers for resistance genes Lr9,LrlO, Lr19, Lr24 were identified in all seven laboratories as amplification products of 1100 bp, 310 bp, 130bp and 310 bp, respectively. The STS markers linked to resistance genes Lr9, LrlO, Lr19, Lr24, Lr29, Lr35 and the SSR marker for Lr39 were robustand highly specific for these genes and will be useful in marker-assisted selection in wheat. However, the amplification product of 378 bp that corresponded with resistance gene Lr28 was detected in all accessions including genotypes lack

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/QC1336" target="_blank" >QC1336: Vývoj nových metod pro monitorování diverzity a hodnocení genových zdrojů a zhodnocení jejich potenciálu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cellular and Molecular Biology Letters

ISSN

1425-8153

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

310

Strana od-do

508-817

Kód UT WoS článku

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EID výsledku v databázi Scopus

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