Kvantitativní stanovení Fusarium culmorum v pletivech pšenice a ječmene pomocí real-time PCR ve srovnání s obsahem DON

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F06%3A1202" target="_blank" >RIV/00027006:_____/06:1202 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of Fusarium culmorum in wheat and barley tissues using real-time PCR in comparison with DON content

Popis výsledku v původním jazyce

A real-time polymerase chain reaction (PCR) assay was developed for the specific detection of Fusarium culmorum in infected seeds. Primers and TaqMan minor groove binder probe were derived from the sequence of a F. culmorum specific PCR product. The specificity of the assay was confirmed by test in seven Fusarium species and 21 non-Fusarium fungal species. With serial dilutions of purified genomic DNA from F. culmorum isolate B as a template, the detection limit of the assay was found to be 0,9 pg of fungal genomic DNA per reaction. A significant correlation (r2=0,982) and collinearity was found between DNA concentration and Ct values of real-time PCR assay with serial diluted DNAs extracted from three seed samples with different DON content. Eight barley and nine wheat varieties infected by F. culmorum isolate B were evaluated in 1 (barley samples) and in 4 (wheat samples) years. The results of real-time PCR analysis and enzyme-linked immunosorbent assay testing for DON content were c

Název v anglickém jazyce

Quantification of Fusarium culmorum in wheat and barley tissues using real-time PCR in comparison with DON content

Popis výsledku anglicky

A real-time polymerase chain reaction (PCR) assay was developed for the specific detection of Fusarium culmorum in infected seeds. Primers and TaqMan minor groove binder probe were derived from the sequence of a F. culmorum specific PCR product. The specificity of the assay was confirmed by test in seven Fusarium species and 21 non-Fusarium fungal species. With serial dilutions of purified genomic DNA from F. culmorum isolate B as a template, the detection limit of the assay was found to be 0,9 pg of fungal genomic DNA per reaction. A significant correlation (r2=0,982) and collinearity was found between DNA concentration and Ct values of real-time PCR assay with serial diluted DNAs extracted from three seed samples with different DON content. Eight barley and nine wheat varieties infected by F. culmorum isolate B were evaluated in 1 (barley samples) and in 4 (wheat samples) years. The results of real-time PCR analysis and enzyme-linked immunosorbent assay testing for DON content were c

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/QG50076" target="_blank" >QG50076: Analýza původců klasových fuzarióz na pšenici na území ČR, vymezení vlivu napadení na hygienickou kvalitu zrna a zajištění účinnější ochrany porostů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Phytopathology

ISSN

0931-1785

e-ISSN

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Svazek periodika

154

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

603-611

Kód UT WoS článku

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EID výsledku v databázi Scopus

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