Mechanism of Plum pox virus resistance in transgenic Prunus domestica L.

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mechanism of Plum pox virus resistance in transgenic Prunus domestica L.

Popis výsledku v původním jazyce

RNA silencing (posttranscriptional gene silencing-PTGS in plant) is a sequence-specific RNA degradation mechanism first discovered in transgenic plants functions as an adaptive immune system targeted against viruses. The hal1marks of RNA silencing, siRNAs (21 nt short and 27 nt long) were detected in transgenic plum, Prunus domestica clone C-5, resistant to Plum pox virus (PPV). The duplex siRNA (21-27-nt) were detected in non-inoculated healthy C5 plums. No such siRNAs were detected in healthy susceptible transgenic plum clone C-6 plums. When C6 plants were inoculated with PPV the 21-nt siRNA was clearly present. Similar short of21 nt was detected in a PPV infected C-5 (a single C5 plum was infected with PPV out of 30 PPV inoculated plants). However,PPV inoculated virus free clone C-5 showed the double banding pattern of siRNA (21-27-nt) as non-inoculated C5. The association of short (21 nt) and long (27nt) siRNA with PPV resistance is correspond with reports in herbaceous plant mode

Název v anglickém jazyce

Mechanism of Plum pox virus resistance in transgenic Prunus domestica L.

Popis výsledku anglicky

RNA silencing (posttranscriptional gene silencing-PTGS in plant) is a sequence-specific RNA degradation mechanism first discovered in transgenic plants functions as an adaptive immune system targeted against viruses. The hal1marks of RNA silencing, siRNAs (21 nt short and 27 nt long) were detected in transgenic plum, Prunus domestica clone C-5, resistant to Plum pox virus (PPV). The duplex siRNA (21-27-nt) were detected in non-inoculated healthy C5 plums. No such siRNAs were detected in healthy susceptible transgenic plum clone C-6 plums. When C6 plants were inoculated with PPV the 21-nt siRNA was clearly present. Similar short of21 nt was detected in a PPV infected C-5 (a single C5 plum was infected with PPV out of 30 PPV inoculated plants). However,PPV inoculated virus free clone C-5 showed the double banding pattern of siRNA (21-27-nt) as non-inoculated C5. The association of short (21 nt) and long (27nt) siRNA with PPV resistance is correspond with reports in herbaceous plant mode

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of Proceedings of the "Eufrin Plum and Prune Working Group Meeting"

ISBN

80-902636-9-0

ISSN

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e-ISSN

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Počet stran výsledku

9

Strana od-do

61-69

Název nakladatele

Research and Breeding Institute of Pomology Holovousy Ltd.

Místo vydání

Holovousy

Místo konání akce

Hradec Králové - Holovousy

Datum konání akce

31. 8. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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