Degradace alergenu Bla g 2: Vzrůstá množství alergenu po částečné mikrobiální degradaci exkrementů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F06%3A1719" target="_blank" >RIV/00027006:_____/06:1719 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Degradation of cockroach allergen Bla g 2: Does the amount of the allergen increase after partial microbial degradation of faeces?

Popis výsledku v původním jazyce

Microbial degradation of German cockroach (Blattella germanica L.) faeces was observed under laboratory conditions at 15oC and 30oC during 84 and 168 days. The degree of degradation and microbial growth were visualized by scanning electron microscopy andby biochemical analyses and correlated to the amount of the major allergen Bla g 2. In degraded and control faeces just after defaecation, the amount of Bla g 2 was assayed by commercial ELISA kit. In addition the total proteins, &#945;-amylase, &#945;and &#946; glucosidases activities were compared. The amount of total proteins as well as enzymatic activities decreased in the extracts from degraded faeces. Altogether with the visualization of fungal decomposers by SEM it indicates the microbial degradation of faeces and denaturation of the protein components depend on temperature. On the contrary, the amount of Bla g 2 after faeces degradation was negatively correlated to the degree of faeces hydrolyses. We hypothesize that microbial

Název v anglickém jazyce

Degradation of cockroach allergen Bla g 2: Does the amount of the allergen increase after partial microbial degradation of faeces?

Popis výsledku anglicky

Microbial degradation of German cockroach (Blattella germanica L.) faeces was observed under laboratory conditions at 15oC and 30oC during 84 and 168 days. The degree of degradation and microbial growth were visualized by scanning electron microscopy andby biochemical analyses and correlated to the amount of the major allergen Bla g 2. In degraded and control faeces just after defaecation, the amount of Bla g 2 was assayed by commercial ELISA kit. In addition the total proteins, &#945;-amylase, &#945;and &#946; glucosidases activities were compared. The amount of total proteins as well as enzymatic activities decreased in the extracts from degraded faeces. Altogether with the visualization of fungal decomposers by SEM it indicates the microbial degradation of faeces and denaturation of the protein components depend on temperature. On the contrary, the amount of Bla g 2 after faeces degradation was negatively correlated to the degree of faeces hydrolyses. We hypothesize that microbial

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/1B53040" target="_blank" >1B53040: Detekce kontaminace potravinářských komodit skladištními členovci pomocí imunochemických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the "9th International Working Conference on Stored Product Protection", 15-18 October 2006

ISBN

85-60234-00-4

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

1108-1113

Název nakladatele

ABRAPOS, Passo Fundo, RS, Brazil

Místo vydání

Sao Paulo, Brazil

Místo konání akce

Campinas, S&#227; Sao Paulo, Brazil

Datum konání akce

15. 10. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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