Embryogenní responzibilita vybraných genotypů Brassica carinata A. Braun v mikrosporové kultuře

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F07%3A2327" target="_blank" >RIV/00027006:_____/07:2327 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Embryogenic responsibility of selected genotypes of Brassica carinata A. Braun to microspore culture

Popis výsledku v původním jazyce

Microspore culture has been used to produce desirable meiotic recombinants in numerous Brassica species. However, the utilization of these recombinants relies on inefficient genome doubling procedures to generate fertile doubled haploids plants. Therefore, it represents a simple and rapid procedure to generate fertile doubled haploids in Brassica carinata. The effects of colchicine, trifluralin and oryzalin on microtubule depolymerization and chromosome doubling in embryogenic microspore cultures of Brassica carinata genotypes were examined. The direct application of these microtubule inhibitors to microspore cultures resulted in the recovery of fertile doubled haploids plants. The application of 10?M trifluralin during 24hrs of microspore culture wasfound to be the superior method for doubled haploid production. Similarly, all tested concentrations of colchicine and oryzalin significantly increased doubled haploid production in comparison with control variants.

Název v anglickém jazyce

Embryogenic responsibility of selected genotypes of Brassica carinata A. Braun to microspore culture

Popis výsledku anglicky

Microspore culture has been used to produce desirable meiotic recombinants in numerous Brassica species. However, the utilization of these recombinants relies on inefficient genome doubling procedures to generate fertile doubled haploids plants. Therefore, it represents a simple and rapid procedure to generate fertile doubled haploids in Brassica carinata. The effects of colchicine, trifluralin and oryzalin on microtubule depolymerization and chromosome doubling in embryogenic microspore cultures of Brassica carinata genotypes were examined. The direct application of these microtubule inhibitors to microspore cultures resulted in the recovery of fertile doubled haploids plants. The application of 10?M trifluralin during 24hrs of microspore culture wasfound to be the superior method for doubled haploid production. Similarly, all tested concentrations of colchicine and oryzalin significantly increased doubled haploid production in comparison with control variants.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agricultura Tropica et Subtropica

ISSN

0231-5742

e-ISSN

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Svazek periodika

40

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

35-38

Kód UT WoS článku

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EID výsledku v databázi Scopus

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