Porovnání detekčních metod pro roztoče Acarus siro (Acari: Acaridida: Acarididae) v obilí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F07%3A2420" target="_blank" >RIV/00027006:_____/07:2420 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain

Popis výsledku v původním jazyce

Acarus siro L. (Acari: Acaridida: Acarididae) is an important pest of stored grain because it contaminates the grain by allergens and transfers pathogenous microorganisms. Rapid detection of contamination enables to intercept an early grain infestation by the pest. In this study, we compared the usability and efciency of various detection approaches. Under laboratory conditions, grain samples of various sizes were infested by different levels of the following contaminants: eggs, adults, and feces of A.siro. The samples were analyzed by enzyme-linked immunosorbent essay (ELISA) by using anti-A. siro polyclonal antibody (immunochemical method), extracted in Berlese-Tullgren funnels, sieved, and processed by the flotation (conventional methods). The adults or juveniles of A. siro could be detected by all the three tested conventional methods and ELISA with detection limits in the range from 221 to 1,157 mites/kg grain. Eggs were detected by the flotation only; the detection limit was 1,9

Název v anglickém jazyce

Comparison of Detection Methods for Acarus siro (Acari:Acaridida: Acarididae) Contamination in Grain

Popis výsledku anglicky

Acarus siro L. (Acari: Acaridida: Acarididae) is an important pest of stored grain because it contaminates the grain by allergens and transfers pathogenous microorganisms. Rapid detection of contamination enables to intercept an early grain infestation by the pest. In this study, we compared the usability and efciency of various detection approaches. Under laboratory conditions, grain samples of various sizes were infested by different levels of the following contaminants: eggs, adults, and feces of A.siro. The samples were analyzed by enzyme-linked immunosorbent essay (ELISA) by using anti-A. siro polyclonal antibody (immunochemical method), extracted in Berlese-Tullgren funnels, sieved, and processed by the flotation (conventional methods). The adults or juveniles of A. siro could be detected by all the three tested conventional methods and ELISA with detection limits in the range from 221 to 1,157 mites/kg grain. Eggs were detected by the flotation only; the detection limit was 1,9

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

—

Projekt

<a href="/cs/project/QF4071" target="_blank" >QF4071: Komplexní metoda monitorování a detekce kontaminace skladovaných obilovin a cereálních produktů škůdci pomocí fyzikálně-chemických, imunochemických a molekulárních technik.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Economic Entomology

ISSN

0022-0493

e-ISSN

—

Svazek periodika

100

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1928-1937

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—