Exprese dehydrinu 5 v průběhu vývoje mrazové odolnosti u ječmene setého (Hordeum vulgare)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F08%3A00000240" target="_blank" >RIV/00027006:_____/08:00000240 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/08:00319151 RIV/62156489:43210/08:00152916

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of dehydrin 5 during the development of frost tolerance in barley (Hordeum vulgare)

Popis výsledku v původním jazyce

Dhn5 gene is the major cold-inducible dehydrin gene in barley. This study deals with the relationship between Dhn5 gene expression and its protein product accumulation, and the development of frost tolerance (FT) upon cold acclimation (CA) in ten barleycultivars of different growth habits and geographical origins. The activation of Dhn5 gene expression was determined by qRT PCR, the accumulation of DHN5 protein was evaluated by protein gel blot analysis and the acquired level of FT was determined by direct frost test. During the first two weeks of CA, there was a rapid increase in Dhn5 gene expression, DHN5 protein accumulation and FT in all cultivars examined. After three weeks of CA, the amount of accumulated DHN5 corresponded with the level of FT of individual cultivars. It can be concluded that the amount of accumulated DHN5 after a certain period of CA differed according to the growth habits of cultivars and can be used as a marker for determination of FT in barley.

Název v anglickém jazyce

Expression of dehydrin 5 during the development of frost tolerance in barley (Hordeum vulgare)

Popis výsledku anglicky

Dhn5 gene is the major cold-inducible dehydrin gene in barley. This study deals with the relationship between Dhn5 gene expression and its protein product accumulation, and the development of frost tolerance (FT) upon cold acclimation (CA) in ten barleycultivars of different growth habits and geographical origins. The activation of Dhn5 gene expression was determined by qRT PCR, the accumulation of DHN5 protein was evaluated by protein gel blot analysis and the acquired level of FT was determined by direct frost test. During the first two weeks of CA, there was a rapid increase in Dhn5 gene expression, DHN5 protein accumulation and FT in all cultivars examined. After three weeks of CA, the amount of accumulated DHN5 corresponded with the level of FT of individual cultivars. It can be concluded that the amount of accumulated DHN5 after a certain period of CA differed according to the growth habits of cultivars and can be used as a marker for determination of FT in barley.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Plant Physiology

ISSN

0176-1617

e-ISSN

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Svazek periodika

165

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000258388300003

EID výsledku v databázi Scopus

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