Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR

Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F10%3A00001035" target="_blank" >RIV/00027006:_____/10:00001035 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Jazyk výsledku
angličtina
Název v původním jazyce
Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR
Popis výsledku v původním jazyce
A quantitative PCR, RT-qPCR, and one-step RT-qPCR for reliable detection and relative quantitation of Prune dwarf virus (PDV) in stone fruits are described. Two plant-expressed genes, actin and 18S rRNA, were used as housekeeping genes for accurate quantitation of PDV in stone fruit trees. The results of this study support the need to use more than one housekeeping gene as an internal control pro reliable quantification of PDV.
Název v anglickém jazyce
Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR
Popis výsledku anglicky
A quantitative PCR, RT-qPCR, and one-step RT-qPCR for reliable detection and relative quantitation of Prune dwarf virus (PDV) in stone fruits are described. Two plant-expressed genes, actin and 18S rRNA, were used as housekeeping genes for accurate quantitation of PDV in stone fruit trees. The results of this study support the need to use more than one housekeeping gene as an internal control pro reliable quantification of PDV.

Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GF - Choroby, škůdci, plevely a ochrana rostlin
OECD FORD obor
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Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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