Invariance of the glass transition temperature of plant vitrification solutions with cooling rate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F12%3A00002489" target="_blank" >RIV/00027006:_____/12:00002489 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cryobiol.2012.07.027" target="_blank" >http://dx.doi.org/10.1016/j.cryobiol.2012.07.027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2012.07.027" target="_blank" >10.1016/j.cryobiol.2012.07.027</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Invariance of the glass transition temperature of plant vitrification solutions with cooling rate

Popis výsledku v původním jazyce

The present work endeavours to increase knowledge in this area characterizing the calorimetric glass transition of the most common plant vitrification solutions, under a wide range of cooling rates. The solutions studied were Plant Vitrification Solutions 1, 2 and 3. Cooling was performed using the calorimeter control or for higher rates, by quenching closed pans with PVS in LN, either naked or inside cryovials. Quenched pans were then transferred to the pre-cooled sample chamber. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145 °C to room temperature, at standard warming rate: 10 °C min-1. Glass transitions showed clear and consistent temperature differences among vitrification solutions, related to composition and water content. Roughly, two sets of Tg values were obtained, for PVS1 and 2, at -112 °C and -114 °C, respectively, and for PSV3, at -90 °C. The observed Tg did not significantly change within a wid

Název v anglickém jazyce

Invariance of the glass transition temperature of plant vitrification solutions with cooling rate

Popis výsledku anglicky

The present work endeavours to increase knowledge in this area characterizing the calorimetric glass transition of the most common plant vitrification solutions, under a wide range of cooling rates. The solutions studied were Plant Vitrification Solutions 1, 2 and 3. Cooling was performed using the calorimeter control or for higher rates, by quenching closed pans with PVS in LN, either naked or inside cryovials. Quenched pans were then transferred to the pre-cooled sample chamber. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145 °C to room temperature, at standard warming rate: 10 °C min-1. Glass transitions showed clear and consistent temperature differences among vitrification solutions, related to composition and water content. Roughly, two sets of Tg values were obtained, for PVS1 and 2, at -112 °C and -114 °C, respectively, and for PSV3, at -90 °C. The observed Tg did not significantly change within a wid

Druh

O - Ostatní výsledky

CEP obor

BJ - Termodynamika

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů