Proteome Analysis of Cold Response in Spring and Winter Wheat (Triticum aestivum) Crowns Reveals Similarities in Stress Adaptation and Differences in Regulatory Processes between the Growth Habits

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F13%3A00002710" target="_blank" >RIV/00027006:_____/13:00002710 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/13:00421944

Výsledek na webu

<a href="http://dx.doi.org/10.1021/pr400600g" target="_blank" >http://dx.doi.org/10.1021/pr400600g</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/pr400600g" target="_blank" >10.1021/pr400600g</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteome Analysis of Cold Response in Spring and Winter Wheat (Triticum aestivum) Crowns Reveals Similarities in Stress Adaptation and Differences in Regulatory Processes between the Growth Habits

Popis výsledku v původním jazyce

A proteomic response to cold treatment (4 °C) has been studied in crowns of a frost-tolerant winter wheat cultivar Samanta and a frost-sensitive spring wheat cultivar Sandra after short-term (3 days) and long-term (21 days) cold treatments. Densitometricanalysis of 2-D differential in gel electrophoresis (2D-DIGE) gels has resulted in the detection of 386 differentially abundant protein spots, which reveal at least a two-fold change between experimental variants. Of these, 58 representative protein spots have been selected for MALDI-TOF/TOF identification, and 36 proteins have been identified. The identified proteins with an increased relative abundance upon cold in both growth habits include proteins involved in carbohydrate catabolism (glycolysis enzymes), redox metabolism (thioredoxin-dependent peroxidase), chaperones, as well as defense-related proteins (protein revealing similarity to thaumatin). Proteins exhibiting a cold-induced increase in the winter cultivar include proteins

Název v anglickém jazyce

Proteome Analysis of Cold Response in Spring and Winter Wheat (Triticum aestivum) Crowns Reveals Similarities in Stress Adaptation and Differences in Regulatory Processes between the Growth Habits

Popis výsledku anglicky

A proteomic response to cold treatment (4 °C) has been studied in crowns of a frost-tolerant winter wheat cultivar Samanta and a frost-sensitive spring wheat cultivar Sandra after short-term (3 days) and long-term (21 days) cold treatments. Densitometricanalysis of 2-D differential in gel electrophoresis (2D-DIGE) gels has resulted in the detection of 386 differentially abundant protein spots, which reveal at least a two-fold change between experimental variants. Of these, 58 representative protein spots have been selected for MALDI-TOF/TOF identification, and 36 proteins have been identified. The identified proteins with an increased relative abundance upon cold in both growth habits include proteins involved in carbohydrate catabolism (glycolysis enzymes), redox metabolism (thioredoxin-dependent peroxidase), chaperones, as well as defense-related proteins (protein revealing similarity to thaumatin). Proteins exhibiting a cold-induced increase in the winter cultivar include proteins

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F09%2F2058" target="_blank" >GA522/09/2058: Dynamika hladin rostlinných hormonů a proteomu během aklimace na chlad u ozimé a jarní pšenice a vybraných rekombinantů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF PROTEOME RESEARCH

ISSN

1535-3893

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

19

Strana od-do

4830-4848

Kód UT WoS článku

000326615000015

EID výsledku v databázi Scopus

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