Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F14%3A00002888" target="_blank" >RIV/00027006:_____/14:00002888 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/14:43898073

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jssc.201301292" target="_blank" >http://dx.doi.org/10.1002/jssc.201301292</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201301292" target="_blank" >10.1002/jssc.201301292</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley

Popis výsledku v původním jazyce

The tests were conducted in field conditions at the Crop Research Institute in Prague?Ruzyně. Each barley genotype was sown in a 1 m2 plot at a sowing rate of 450 seeds/m. Within the first set of experiments the following barley varieties were examined:Aktiv, Gladys, Henrike, Lilly, Radegast, Sebastian, Signora, Sladar, and Tolar. Each variety was grown and tested in artificially infected and control variants. Artificially infected samples were inoculated at the beginning of heading using an isolate mixture from F. culmorum isolates.Within the first phase of this methodological study, we focused on the sample preparation for the analysis of the broadest possible spectrum of metabolites/small molecules isolated from the barley tissue. During the courseof our experiments, 1 g of barley grain samples (a pool of control and infected barley of variety Sebastian) was homogenized using an Ultraturrax with 10 mL of different solvents or mixtures of solvents with decreasing polarity. Specific

Název v anglickém jazyce

Rapid LC-MS-based metabolomics method to study the Fusarium infection of barley

Popis výsledku anglicky

The tests were conducted in field conditions at the Crop Research Institute in Prague?Ruzyně. Each barley genotype was sown in a 1 m2 plot at a sowing rate of 450 seeds/m. Within the first set of experiments the following barley varieties were examined:Aktiv, Gladys, Henrike, Lilly, Radegast, Sebastian, Signora, Sladar, and Tolar. Each variety was grown and tested in artificially infected and control variants. Artificially infected samples were inoculated at the beginning of heading using an isolate mixture from F. culmorum isolates.Within the first phase of this methodological study, we focused on the sample preparation for the analysis of the broadest possible spectrum of metabolites/small molecules isolated from the barley tissue. During the courseof our experiments, 1 g of barley grain samples (a pool of control and infected barley of variety Sebastian) was homogenized using an Ultraturrax with 10 mL of different solvents or mixtures of solvents with decreasing polarity. Specific

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

—

Projekt

<a href="/cs/project/QI111B044" target="_blank" >QI111B044: Komplexní strategie pro minimalizaci negativního dopadu infekce toxikogenními houbami r. Fusarium v obilovinách a odvozených produktech.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF SEPARATION SCIENCE

ISSN

1615-9306

e-ISSN

—

Svazek periodika

37

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

912-919

Kód UT WoS článku

000334059900003

EID výsledku v databázi Scopus

—