Point mutations in the sodium channel gene conferring tau-fluvalinate resistance in Varroa destructor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F14%3A00002891" target="_blank" >RIV/00027006:_____/14:00002891 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/ps.3679" target="_blank" >http://dx.doi.org/10.1002/ps.3679</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ps.3679" target="_blank" >10.1002/ps.3679</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Point mutations in the sodium channel gene conferring tau-fluvalinate resistance in Varroa destructor

Popis výsledku v původním jazyce

Background: Sodium channels (SC) in mites and insects are target sites for pesticides including pyrethroids. Point mutations of SC gene were reported to change structural conformation of the protein and sensitivity to pesticides. To find mutations in SCgene of Varroa destructor (VmNa) we analyzed VmNa gene sequences available in GenBank and prepared specific primers for amplification of two fragments harboring the regions coding for (i) IIS4-IIS6 in positions 2837-3337 and (ii) IIIS4-3´terminus in positions 4737-6500 according to VmNa cDNA sequence AY259834. Results: Sensitive and resistant populations did not differ in amino acid sequences of the IIIS4-3´terminus VmNa region. The differences were found in the fragment IIS4-IIS6: In resistant population, mutation C(3004)>G resulted in substitution L(1002)> (codon ctg > gtg) in the position equivalent to L925 in house fly in the linker connecting segments IIS4 and IIS5. Additionally, mutation F(1052) >L (codon ttc > ctc) in the positio

Název v anglickém jazyce

Point mutations in the sodium channel gene conferring tau-fluvalinate resistance in Varroa destructor

Popis výsledku anglicky

Background: Sodium channels (SC) in mites and insects are target sites for pesticides including pyrethroids. Point mutations of SC gene were reported to change structural conformation of the protein and sensitivity to pesticides. To find mutations in SCgene of Varroa destructor (VmNa) we analyzed VmNa gene sequences available in GenBank and prepared specific primers for amplification of two fragments harboring the regions coding for (i) IIS4-IIS6 in positions 2837-3337 and (ii) IIIS4-3´terminus in positions 4737-6500 according to VmNa cDNA sequence AY259834. Results: Sensitive and resistant populations did not differ in amino acid sequences of the IIIS4-3´terminus VmNa region. The differences were found in the fragment IIS4-IIS6: In resistant population, mutation C(3004)>G resulted in substitution L(1002)> (codon ctg > gtg) in the position equivalent to L925 in house fly in the linker connecting segments IIS4 and IIS5. Additionally, mutation F(1052) >L (codon ttc > ctc) in the positio

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/QI111A119" target="_blank" >QI111A119: Identifikace příčin a metody prevence ztrát včelstev</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pest Management Science

ISSN

1526-498X

e-ISSN

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Svazek periodika

70

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

889-894

Kód UT WoS článku

000335565000005

EID výsledku v databázi Scopus

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