Reliable detection of European stone fruit yellows phytoplasma in apricot and peach trees

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Reliable detection of European stone fruit yellows phytoplasma in apricot and peach trees

Popis výsledku v původním jazyce

To evaluate most reliable method for European stone fruit yellows (ESFY) phytoplasma detection, apricot and peach trees infected with ESFY were evaluated for symptom expression. Biological indexing was carried out on the peach indicator GF-305 and molecular detection by PCR was carried out with different primer pairs. Some false positive reactions in nested PCR assays were obtained, but also the biological indexing was not reliable because of many negative results from symptomatic trees. The presence ofspecific and typical symptoms on leaves is still the most reliable criterion for detection of ESFY in apricot and peach trees grown in the Czech Republic.

Název v anglickém jazyce

Reliable detection of European stone fruit yellows phytoplasma in apricot and peach trees

Popis výsledku anglicky

To evaluate most reliable method for European stone fruit yellows (ESFY) phytoplasma detection, apricot and peach trees infected with ESFY were evaluated for symptom expression. Biological indexing was carried out on the peach indicator GF-305 and molecular detection by PCR was carried out with different primer pairs. Some false positive reactions in nested PCR assays were obtained, but also the biological indexing was not reliable because of many negative results from symptomatic trees. The presence ofspecific and typical symptoms on leaves is still the most reliable criterion for detection of ESFY in apricot and peach trees grown in the Czech Republic.

Druh

C - Kapitola v odborné knize

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Phytoplasmas and phytoplasma disease management: how to reduce their economic impact

ISBN

978-88-909922-0-9

Počet stran výsledku

7

Strana od-do

190-196

Počet stran knihy

287

Název nakladatele

IPWG - International Phytoplasmologist Working Group

Místo vydání

Italy

Kód UT WoS kapitoly

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