Two-dimensional gel proteomic analysis of Dermatophagoides farinae feces

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F15%3A00003047" target="_blank" >RIV/00027006:_____/15:00003047 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10493-014-9848-1" target="_blank" >http://dx.doi.org/10.1007/s10493-014-9848-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10493-014-9848-1" target="_blank" >10.1007/s10493-014-9848-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Two-dimensional gel proteomic analysis of Dermatophagoides farinae feces

Popis výsledku v původním jazyce

In this study, we prepared and using proteomic methods analyzed a D. farinae fecal extract for the first time. This study contains in-depth proteomic mapping of the allergenic isoforms from the D. farinae fecal extract. Despite extensive analysis, MALDITOF/TOF spectrometry showed that only six proteins/allergens, Der f1, Der f2, Der f3, Der f6, Der f15 and ferritin, originated from D. farinae. No other analyzed proteins were exactly assigned to Dermatophagoides or to similar invertebrate species by sequence similarity. Isoforms of the allergens Der f1, Der f3 and Der f15 were identified in more MWs indicating the presence of zymogens and active-enzyme forms. The identified fecal allergens accumulate in the environment during the life of the mite and represent quantitatively greater amounts of mite immunogens than those that were missed in the 2D-E. The results contribute to our understanding of D. farinae digestive physiology with regard to the enzymes/proteins present in the feces.

Název v anglickém jazyce

Two-dimensional gel proteomic analysis of Dermatophagoides farinae feces

Popis výsledku anglicky

In this study, we prepared and using proteomic methods analyzed a D. farinae fecal extract for the first time. This study contains in-depth proteomic mapping of the allergenic isoforms from the D. farinae fecal extract. Despite extensive analysis, MALDITOF/TOF spectrometry showed that only six proteins/allergens, Der f1, Der f2, Der f3, Der f6, Der f15 and ferritin, originated from D. farinae. No other analyzed proteins were exactly assigned to Dermatophagoides or to similar invertebrate species by sequence similarity. Isoforms of the allergens Der f1, Der f3 and Der f15 were identified in more MWs indicating the presence of zymogens and active-enzyme forms. The identified fecal allergens accumulate in the environment during the life of the mite and represent quantitatively greater amounts of mite immunogens than those that were missed in the 2D-E. The results contribute to our understanding of D. farinae digestive physiology with regard to the enzymes/proteins present in the feces.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental and Applied Acarology

ISSN

0168-8162

e-ISSN

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Svazek periodika

65

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

73-87

Kód UT WoS článku

000345345200006

EID výsledku v databázi Scopus

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