In vivo and in vitro effectiveness of Azadirachta indica-synthesized silver nanocrystals against Plasmodium berghei and Plasmodium falciparum, and their potential against malaria mosquitoes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F16%3A00003653" target="_blank" >RIV/00027006:_____/16:00003653 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.rvsc.2016.03.001" target="_blank" >http://dx.doi.org/10.1016/j.rvsc.2016.03.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.rvsc.2016.03.001" target="_blank" >10.1016/j.rvsc.2016.03.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vivo and in vitro effectiveness of Azadirachta indica-synthesized silver nanocrystals against Plasmodium berghei and Plasmodium falciparum, and their potential against malaria mosquitoes

Popis výsledku v původním jazyce

In this research, we synthesized silver nanoparticles (AgNP) using the Azadirachta indica seed kernel extract as reducing and stabilizing agent. AgNP were characterized by UV–vis spectrophotometry, SEM, EDX, XRD and FTIR spectroscopy. The A. indica seed kernel extract was toxic against Anopheles stephensi larvae and pupae, LC50 were 232.8 ppm (larva I), 260.6 ppm (II), 290.3 ppm (III), 323.4 ppm (IV), and 348.4 ppm (pupa). AgNP LC50 were 3.9 ppm (I), 4.9 ppm (II), 5.6 ppm (III), 6.5 ppm (IV), and 8.2 ppm (pupa). The antiplasmodial activity of A. indica seed kernel extract and AgNP was evaluated against CQ-resistant (CQ-r) and CQ-sensitive (CQ-s) strains of Plasmodium falciparum. IC50 of A. indica seed kernel extract were 63.18 µg/ml (CQ-s) and 69.24 µg/ml (CQ-r). A. indica seed kernel-synthesized AgNP achieved IC50, of 82.41 µg/ml (CQ-s) and 86.12 µg/ml (CQ-r). However, in vivo anti-plasmodial experiments conducted on Plasmodium berghei infecting albino mice showed moderate activity of the A. indica extract and AgNP. Overall, this study showed that the A. indica-mediated fabrication of AgNP is of interest for a wide array of purposes, ranging from IPM of mosquito vectors to the development of novel and cheap antimalarial drugs.

Název v anglickém jazyce

In vivo and in vitro effectiveness of Azadirachta indica-synthesized silver nanocrystals against Plasmodium berghei and Plasmodium falciparum, and their potential against malaria mosquitoes

Popis výsledku anglicky

In this research, we synthesized silver nanoparticles (AgNP) using the Azadirachta indica seed kernel extract as reducing and stabilizing agent. AgNP were characterized by UV–vis spectrophotometry, SEM, EDX, XRD and FTIR spectroscopy. The A. indica seed kernel extract was toxic against Anopheles stephensi larvae and pupae, LC50 were 232.8 ppm (larva I), 260.6 ppm (II), 290.3 ppm (III), 323.4 ppm (IV), and 348.4 ppm (pupa). AgNP LC50 were 3.9 ppm (I), 4.9 ppm (II), 5.6 ppm (III), 6.5 ppm (IV), and 8.2 ppm (pupa). The antiplasmodial activity of A. indica seed kernel extract and AgNP was evaluated against CQ-resistant (CQ-r) and CQ-sensitive (CQ-s) strains of Plasmodium falciparum. IC50 of A. indica seed kernel extract were 63.18 µg/ml (CQ-s) and 69.24 µg/ml (CQ-r). A. indica seed kernel-synthesized AgNP achieved IC50, of 82.41 µg/ml (CQ-s) and 86.12 µg/ml (CQ-r). However, in vivo anti-plasmodial experiments conducted on Plasmodium berghei infecting albino mice showed moderate activity of the A. indica extract and AgNP. Overall, this study showed that the A. indica-mediated fabrication of AgNP is of interest for a wide array of purposes, ranging from IPM of mosquito vectors to the development of novel and cheap antimalarial drugs.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Veterinary Science

ISSN

0034-5288

e-ISSN

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Svazek periodika

106

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

14-22

Kód UT WoS článku

000377923400003

EID výsledku v databázi Scopus

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